摘要
背景:大剂量化疗药物可造成严重的骨髓损伤,除药物治疗外,干细胞移植已越来越多的用于骨髓损伤的治疗。目的:观察人羊膜间充质干细胞移植对顺铂所致骨髓抑制小鼠造血功能恢复的影响。方法:体外培养人羊膜间充质干细胞,雌性ICR小鼠被随机分为3组,除空白组外,其余小鼠腹腔注射顺铂诱导ICR小鼠制备骨髓抑制模型,建模后人羊膜间充质干细胞移植组小鼠尾静脉注射人羊膜间充质干细胞3.0×105/g,空白组和模型组注射等量PBS。结果与结论:连续给予顺铂7d后,ICR小鼠体质量明显降低,但模型组与人羊膜间充质干细胞移植组之间差异无显著性意义。模型组小鼠外周血白细胞、淋巴细胞、单个核细胞、红细胞、血红蛋白、红细胞压积和血小板等水平明显低于空白组(P<0.01),于首次给顺铂后第21天开始恢复。人羊膜间充质干细胞移植组外周血的上述指标较模型组提前7d开始恢复,第21天时,已接近或达到空白组水平;与空白组相比,人羊膜间充质干细胞移植后的一两天及移植后的2周出现了一过性的白细胞增高和血小板数增加。第24天,人羊膜间充质干细胞移植组小鼠股骨髓腔冲洗液有核细胞数明显高于模型组(P<0.05),骨髓组织病理学检查结果也显示,人羊膜间充质干细胞移植组小鼠股骨骨髓组织结构明显改善,与空白组相似,骨髓细胞数明显增多,可见较多的巨核细胞,且人羊膜间充质干细胞移植组后2周,骨髓组织可见分布较广的小鼠抗人核单克隆抗体-FITC阳性的移植细胞定植。以上结果表明,人羊膜间充质干细胞移植治疗较早地改善顺铂所致骨髓抑制小鼠的造血功能。
BACKGROUND： High-dose chemotherapy often results in severe bone marrow damage. Beside medicines, stern cells transplantation has also been as a strategy for the treatment of bone marrow damage. OBJECTIVE： To investigate the effects of human amnion-derived mesenchymal stem cells transplantation on the hematopoietic function of myelosuppression mice induced by cisplatin. METHODS： Human amnion-derived mesenchymal stem cells were cultured in vitro. The female Imprinting Control Region mice were divided into three groups. Excepted for the mice in the blank group, the mice in the other groups were intraperitoneal injected with cisplatin to make the myelosuppression Imprinting Control Region mice, then the 3.0×10^5/g human amnion-derived mesenchymal stem cells were injected into the mice in the human amnion-derived mesenchymal stem cells transplantation group through the caudal vein, and PBS solution in the same dose was injected in the mice of blank group and model group. RESULTS AND CONCLUSION： On day 7, the body weight of Imprinting Control Region mice was reduced after administrated with cispiatin for 7 days, there was no significant difference between model group and human amnion-derived mesenchymal stern cells transplantation group. The number of white blood cells, lymphocytes,mononuclear cells, red blood cells, hemoglobin, hematocrit and platelet of peripheral blood in the model group were significantly lower than those in the blank group （P 〈 0.05）, then the indexes above began to recover on day 21 after cisplatin treatment. The recovery of the indexes above of the peripheral blood in the human amnion-derived mesenchymal stem cells transplantation group was 7 days earlier than that in the model group, and the indexes above were recovered to normal level at 21 days; compared with blank group, the number of white blood cells and platelet in the human amnion-derived mesenchymal stem ceils transplantation group was transiently increased at 1-2 days before transplantation and 2 days after transplantation. At 24 days after transplantation, the number of bone marrow karyocytes in human amnion-derived mesenchymal stem ceils transplantation group was significantly higher than that in the model group （P 〈 0.05）. Bone marrow histopathological testing results showed that the mice femur bone marrow organizational structure in the human amnion-derived mesenchymal stem cells transplantation group was significantly improved. Compared with blank group, the number of bone marrow nucleated cells, especially megakaryocytes, was higher in human amnion-derived mesenchymal stem cells transplantation group, and at 2 weeks after human amnion-derived mesenchymal stem cells transplantation, many mouse anti-human nuclei monoclonal antibody-FITC positive cells were colonized in myeloid tissue. These results showed that human amnion-derived mesenchymal stem ceils transplantation can remarkably ameliorate hematopoietic function in cisplatin induced myelosuppression mice.
出处
《中国组织工程研究》
CAS
CSCD
2013年第23期4248-4255,共8页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(30860338)
贵州省科学技术实验室建设平台(209.001.106.01)项目号~~
关键词
干细胞
干细胞移植
人羊膜间充质干细胞
顺铂
骨髓抑制
造血功能
小鼠
白细胞
红细胞
淋
巴细胞
单个核细胞
血红蛋白
红细胞压积
血小板
国家自然科学基金
干细胞图片文章
stem cells
stem cell transplantation
human amniotic mesenchymal stem cells
cisplatin
bonemarrow suppression
hematopoietic function
mice
white blood cells
red blood cells
lymphocytes
mononuclearcells
hemoglobin
hematocrit
platelet
National Natural Science Foundation of China
stem cellphotographs-containing paper
