摘要
目的进一步了解H5N1禽流感病毒的致病机制,同时对H5N1禽流感病人的临床救治方案提供科学依据。方法采集2011年深圳市1例H5N1禽流感病例病程中多份血液样本,应用流式细胞术和酶联免疫法,检测该病人外周血中各亚群淋巴细胞及细胞因子水平。结果 H5N1禽流感患者外周血中各亚群淋巴细胞均出现绝对数量减少,CD4+/CD8+出现倒置。同时发现血清中高水平的IL-6、IL-10、TNF、IFN-γ、IL-12、IP-10和MCP-1。结论 H5N1禽流感病毒会对机体的免疫系统造成严重伤害,同时会刺激机体过度表达高水平的细胞因子,从而导致典型的免疫应答介导的病理损伤。
In order to understand the pathogenic mechanism of avian influenza virus and provide the scientific basis for effective therapeutic strategies, peripheral blood lymphocyte counts and cytokine of a man infected with H5N1 virus in Shenzhen were detected by flow cytometric analysis and ELISA assay. Compared with those of healthy control, the numbers of peripheral B, NK, CD＋ T, and CD8＋ T cell in patient infected with H5N1 were significantly decreased, and the inverted ratios of CD＋ to CD＋cells was detected. Cytokine analysis showed the high levels of serum IL-6, IL-10, TNF, IFN-γ, IL-12, IP-10, and MCP-1. These findings indicated that H5N1 virus could be the cause of serious damage to body＇s immune system, including cellular immunity and humoral immunity. Cytokines are essential for efficient elimination of virus. However, hyper-activation of inflammatory cytokines may initiate or exacerbate pathological damage in this viral infection.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2013年第7期697-699,共3页
Chinese Journal of Zoonoses
