摘要
建立了大豆分离蛋白中三聚氰胺的液相色谱方法。大豆分离蛋白经过1％三氯乙酸和饱和乙酸铅溶液提取后，经过阳离子交换固相萃取柱净化处理，用5％氨化甲醇洗脱，洗脱液用高效液相色谱紫外检测器检测，外标法定量。三聚氢胺的质量浓度在0．4～40mg／L范围内与色谱峰面积呈良好的线性关系，相关系数为0．9990。方法的检出限为0．6mg／kg，定量限为2mg／kg。2，4，20mg／kg3个浓度水平的加标回收率在78．82％-92．95％范围内。该方法分析成本低，测定结果准确，能满足常规检测及食品安全监控的需要。
Liquid chromatography was developed for determining melamine residues in soy protein isolate. The target compound was extracted by trichloroacetic acid and saturation of lead acetate and cleaned up with cation-exchange solid phase extraction column. The extract was eluted with mixture of amonium methanol （volume ratio was 5 ： 95）. The determination was performed on LC with UV detector and the external calibration standard was used for quantification.The melamine concentration was linear with peak area in the range of 0.4-40 mg / L with r2=0.999 0. The detection limit was 0.6 mg/ kg, the quantification limit of melamine was 2 mg/kg. The recovery at levels of 2,4,20 mg/kg ranged from 78.82% to 92.95%. This method provids accurate results with less time consuming and lower cost, which can be meet the requirements of the routine testing and food safety monitoring.
出处
《化学分析计量》
CAS
2013年第4期69-71,共3页
Chemical Analysis And Meterage
关键词
液相色谱
三聚氰胺
大豆分离蛋白
liquid chromatography
melamine
soy protein isolate
