摘要
目的:分析日本、台湾杂色鲍及其杂交后代("东优1号")足肌和壳的氨基酸含量及组成。方法:采用水解法和磺基水杨酸提取法进行氨基酸分析,以氨基酸比值系数分(SRCAA)进行评价。结果:3种鲍足肌含有18种蛋白质氨基酸和3种非蛋白质氨基酸;其SRCAA均大于80%,且E/T、E/N符合FAO/WHO的蛋白模式;"东优1号"和日本群体足肌的EAA、总氨基酸(TAA)及其他功能性氨基酸的含量均显著高于台湾群体(P<0.05);3种鲍壳蛋白中含有18种氨基酸,"东优1号"鲍壳的TAA和EAA含量均高于双亲。结论:3种鲍足肌蛋白中的氨基酸种类齐全,比例合理,具有较高的营养价值,是一种优质的蛋白源。"东优1号"的足肌和壳在氨基酸营养方面,较好地综合了双亲的优良性状,且部分性状优于双亲。
Objective：The paper aims at analyzing amino acid contents and composition of foot muscle and shell of RITAI Haliotis diversicolor and their reciprocal hybrids.Method：The amino acids were hydrolyzed by acid or base,extracted by sulfosalicylic acid,and at last it is estimated by the score of ratio coefficient of amino acid.Result：The foot muscle of three kinds of Haliotis diversicolor contains eighteen kinds of proteinaceous amino acid and three kinds of nonprotein amino acid.The SRCAA of foot muscle of three kinds of Haliotis diversicolors is all over 80%,and their E/T and E/N are consistent with the FAO/WHO proteinaceous model.The contents of TAA,EAA and other functional amino acids of foot muscle in Dongyou No.1 and Japan population are obviously higher than those of Taiwan population（P0.05）.The protein of shell of Haliotis diversicolors contain eighteen kinds of amino acid.The contents of TAA and EAA of shell in Dongyou No.1 are higher than those of its parents.Conclusion：The foot muscle protein of Haliotis diversicolor has high nutritional value and contains variety of amino acid,which is in reasonable proportions.It is the best source of high-quality protein.The amino acid nutrition of foot muscle and shell of Dongyou No.1 can be improved by hybridization and have some qualities that are superior to its parents.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2013年第6期249-256,共8页
Journal of Chinese Institute Of Food Science and Technology
关键词
杂色鲍
日本群体
台湾群体
东优1号
氨基酸
Haliotis diversicolor
Japan population
Taiwan population
Dongyou No.1
amino acid
