摘要
为了优化山核桃外蒲壳多酚的提取工艺,在乙醇提取方法的基础上,探讨超声波及微波辅助处理对多酚提取率的影响。研究结果表明,超声波辅助提取蒲多酚工艺条件是:60%乙醇,料液比1:20(g/mL),40℃,200 W,30 min,提取1次,在此条件下多酚得率19.87%;微波辅助提取蒲多酚工艺条件是:60%乙醇,料液比1:20(g/mL),40℃,600 W,4 min,提取1次,在此条件下多酚得率为15.39%。将超声提取的多酚进行体外抗氧化分析,结果山核桃蒲多酚对DPPH自由基和超氧阴离子具有较高的清除率。
To enhance the extraction efficiency of polyphenols from Carya cathayensis exoearp,two new methods based on the existing organic solvent extraction method,ultrasonic and microwave-assisted extraction methods,were developed.With 60% aqueous ethanol as extraction agent,the yield of the ultrasonic assisted extraction method and the microwave-assisted extraction method were 19.87% and 15.39% respectively.The conditions for ultrasonic-assisted extraction methods were:solid-liquid ratio of 1:20(g/mL),40 ℃,200 W,30 min,single extraction.The conditions for the microwave-assisted extraction were:solid-liquid ratio of 1:20(g/mL),40 ℃,600 W,4 min.The polyphenols extracted using the ultrasonic method was investigated for its antioxidant activities in vitro.The results showed that the polyphenols extracted exhibited high levels of DPPH and Hydroxyl radical scavenging capacities.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2013年第6期32-39,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省重大科技专项重点农业项目(2009C12033)
浙江省自然科学基金项目(Y2101447)
浙江省公益性项目(2011C22061)
关键词
山核桃
外蒲壳多酚
提取工艺
抗氧化分析
Carya cathayensis
exoearp polyphenols
extraction technology
antioxidant analysis.