摘要
目的探讨呼吸道合胞病毒感染后血浆IL-17和血清IgE水平变化以及IL-17与IgE的关系,以期为早期预防RSV毛细支气管炎向儿童哮喘转化提供新的分子学依据。方法利用酶联免疫吸附试验(ELISA)测定60例毛细支气管炎患儿血清RSV抗体,分为RSV毛支组和非RSV毛支组,并用酶联免疫吸附试验(ELISA)测定该60例毛细支气管炎患儿和20例健康体检婴幼儿外周血浆IL-17水平,并同时检测患儿血清IgE水平。应用SPSS17.0软件处理数据。结果RSV毛支组血浆IL-17水平为17.10±5.15pg/mL,非RSV毛支组血浆IL-17水平为13.60±2.76pg/mL,均高于正常对照组10.78±1.99pg/mL,差异有统计学意义(P<0.05),RSV毛支组血浆IL-17水平与非RSV毛支组比较,差异有统计学意义(P<0.05)。RSV毛支组血清IgE水平为67.81±51.06IU/mL,非RSV毛支组血清IgE水平为57.75±34.76 IU/mL,均较正常对照组18.51±9.44IU/mL升高,差异有统计学意义(P<0.05)。但RSV毛支组血清IgE水平与非RSV毛支组血清IgE水平比较,差异无统计学意义(P>0.05)。RSV毛支组血浆IL-17水平与血清IgE水平呈正相关(r=0.8412)。结论 1.细胞因子IL-17参与了呼吸道合胞病毒感染后的免疫机制.2.毛细支气管炎患儿体内存在类似哮喘的免疫机制,血清IgE的产生与具体病原体无关。3.呼吸道合胞病毒感染后毛细支气管炎患儿血浆IL-17可能促进了血清IgE的产生。
Objective： Explore the variations of IL-17and IgE after respiratory syncytial virus infection and the relationship between IL-17and serum IgE.In order to provide new molecular basis in which the early prevention of RSV bronchiolitis transform to asthma in children.Method： Using enzyme linked immunosorbent assay（ELISA） method to determine RSV antibody of children And so is divided into the RSV bronchiolitis group and non-RSV bronchiolitis group.Using enzyme linked immunosorbent assay（ELISA） method to determine IL-17 and serum IgE in 60 cases of children with bronchiolitis and 20 cases of healthy infants in plasma.useing spss to process date.Result： IL-17 in children with RSV bronchiolitis is 17.47 ± 1.30pg / mL,in non-RSV bronchiolitis group is 13.58 ± 0.89pg / mL and in the control group is 10.57 ± 0.77 pg / mL.They have significant difference（P 0.05）.IL-17 in RSV bronchiolitis group and in non-RSV bronchiolitis group have significant difference（P 0.05）.Serum IgE in children with RSV bronchiolitis is 84.28 ± 15.50IU / mL and in non-RSV bronchiolitis group is73.14 ± 11.70 IU / mL,in the control group is 14.68 ± 1.58 IU / mL.They have significant difference（P 0,05）.But serum IgE in RSV bronchiolitis group and in non-RSV bronchiolitis group does not have difference（P 0.05）.And IL-17 and IgE in serum in children wtith RSV bronchiolitis correlate.Conclusion： 1.IL-17 may participat in the RSV infection immune mechanism.2.Children with bronchiolitis may exist similar immune mechanisms to athma,serum IgE may not be associated with specific pathogens.3.Children with bronchitis after RSV infection IL-17may promote the generation.of serum IgE.
出处
《中国优生与遗传杂志》
2013年第7期130-132,F0003,共4页
Chinese Journal of Birth Health & Heredity
