摘要
目的研究花姜酮对人肝癌SMMC-7721细胞生长侵袭能力的影响及对CXCR4 mRNA和蛋白表达的影响,探讨花姜酮抗肝癌侵袭可能的作用机制。方法分别应用花姜酮10μmol/L、20μmol/L处理人肝癌细胞,采用Transwell小室检测肝癌细胞的侵袭能力,RT-PCR、Western blotting实验检测CXCR4 mRNA及蛋白表达的变化。结果花姜酮处理人肝癌细胞48 h后,对照组细胞透膜数为(56.8±7.8)个/视野,10μmol/L组透膜数为(41.4±4.8)个/视野,20μmol/L组透膜数为(23.2±5.6)个/视野,随花姜酮浓度增加,细胞侵袭人工基底膜的能力显著降低(P<0.05),RT-PCR及Western blotting结果显示CXCR4 mRNA及蛋白表达随花姜酮浓度的增加而降低(P<0.05)。结论花姜酮可能通过降低CXCR4的表达抑制人肝癌SMMC-7721细胞的生长和侵袭能力。
Objective To study the effect of Zerumbone on the invasion ability of human hepatoma SMMC-7721 cell and the expression of CXCR4 mRNA and protein, and to explore the possible mechanisms of Zerumbone＇ s anti-tumor invasion ability. Methods Human hepatocellular carcinoma cells were treated with Zerumbone （10 μmol/L, 20 μmol/ L）. Transwell chambers were used to detect the invasion ability of hepatocarcinoma. RT-PCR and Western blotting were adopted to detect the expression of CXCR4 mRNA and protein respectively. Results After 48 hours of Zerumbone treat- ment, the numbers of membrane-permeating ceils in the control group averaged to （56.8 ± 7.8） per field, and those in 10 μmol/L and 20 μmol/L Zerumbone groups were （41.4 ± 4.8） and （23.2 ± 5.6） per field respectively. With the increasing of Zerumbone concentration, the ability of human heptoma cells invading reconstituted basement membrane was decreased significantly （P 〈 0.05 ）. The results of RT-PCR and Western blotting showed decrease of CXCR4 mRNA and protein expression （P 〈 0.05）. Conclusion Zerumbone may reduce the growth and invasive ability of human heparoma SMMC-7721 cells by reducing the expression of CXCR4 mRNA and protein.
出处
《胃肠病学和肝病学杂志》
CAS
2013年第7期624-627,共4页
Chinese Journal of Gastroenterology and Hepatology
