摘要
目的 检测正常月经周期子宫内膜和多囊卵巢综合征（PCOS）患者子宫内膜上肾素-血管紧张素系统（RAS）各组分的表达.方法 选取正常月经周期子宫内膜,其中增殖期和分泌期各30例,采用免疫组化SP法检测AngⅡ、AT1R、AT2R、ACE2、Ang-（1-7）、Mas在子宫内膜蛋白水平的表达.收集PCOS患者增殖期子宫内膜15例,正常增殖期子宫内膜15例.常规HE染色判定子宫内膜标本的组织学分期；实时荧光相对定量聚合酶链反应（RQ-PCR）检测AT1R、AT2R、ACE2及Mas在子宫内膜分子水平的表达.结果 正常月经周期子宫内膜AngⅡAT1R、AT2R、ACE2、Ang-（1-7）及Mas的表达呈周期性改变,趋势相似,腺上皮的表达强于间质,分泌期强于增殖期.PCOS患者增殖期子宫内膜中ACE2 mRNA、AT1R mRNA、AT2R mRNA、Mas mRNA明显高于对照组（P＜0.01）.结论 宫内膜局部存在RAS系统,其各组分存在着周期性变化.PCOS患者子宫内膜中AT1R、AT2R、ACE2、Mas分子水平表达增强.
Objective To explore the expression of renin-angiotensin system component （ACE-Ang Ⅱ-AT1/AT2 and ACE2-Ang-（1-7）-Mas） in endometrium of polycystic ovary syndrome （PCOS） patients and normal controls.Methods Thirty cases of normal endometrium in proliferative and secretory phases respectively were selected for the protein levels of Ang Ⅱ,AT1,AT2,ACE2,Ang-（1-7） and Mas through immunohistochemistry.And the specimens of proliferative endometrium from 15 PCOS patients and 15 normal controls respectively were prepared for the expressions of AT1,AT2,ACE2 and Mas mRNA through relative quantitative-polymerase chain reaction （RQ-PCR）.The histological phases of endometria were confirmed by hematoxylin and eosin staining under microscope.Results The expressions of Ang Ⅱ,AT1R,AT2R,ACE2,Ang-（1-7） and Mas showed periodical changes in endometrium throughout normal menstrual cycles and shared a similar trend.The expression was more pronounced in epithelial cells than that in stromal cells while it was also more dramatic in secretory phase than proliferative phase; the mRNA expressions of AT1,AT2,ACE2 and MAS were higher in PCOS endometrium than those in normal controls.Statistically significant differences existed between two groups （P 〈 0.01）.Conclusion There is local existence of RAS in endometrium; increased expressions of AT1 mRNA,AT2 mRNA,ACE2 mRNA and Mas mRNA in endometrium of PCOS may influence endometrial development and play a role in the pathological process of PCOS.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2013年第25期1989-1992,共4页
National Medical Journal of China
