摘要
植物叶际有着丰富的生物多样性,是一个复杂的微生态系统,叶际表面微生物通过与宿主植物的互作,改善其栖居环境并促进植物生长。从芹菜叶际生态系统中分离得到吡哆醛还原酶(Pyridoxal Reductase,PLR),采用苯肼衍生方法检测其活性并确定PLR的来源,对叶际表面微生物进行分离、计数,确定储藏过程中芹菜叶片表面微生物的变化情况,并对PLR的基本酶学性质进行初步研究。结果表明,PLR主要来源于真菌,在相同菌浓度下,PLR活性的强弱依次为真菌>细菌>放线菌。该酶的最适温度为37℃,最适反应pH值为7.5,并且在NADPH的参与下,其催化活性显著提高。
The phyllosphere is a complicated microecological system where colonized by a variety of Microorganism. The epiphytes could improve the dwelling environment and promote the growth of plant through interaction with the host plant. The Pyridoxal Reduetase （PLR）isolated from ecosystem of celery leaf, and determine the sources of the PLR with the phenylhydrazine derivative method. Separation and counts of the microorganism separated from celery leaf, determine the changes of mieroorgenism at the different storage condition, and study on the enzymatic properties of PLR. The results showed that PLR was mainly derived from the fungus of celery leaf surface. The intensity of the PLR activity was fungi〉bacteria〉 actinomycetes at the same bacteria concentration. The enzyme had an optimal temperature and pH at 37℃ and 7.5. PLR showed a higher catalytic activity of reduction of PL with NADPH.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第11期25-28,共4页
Guangdong Agricultural Sciences
关键词
芹菜
叶际微生物
吡哆醛还原酶
酶性质
celery
phyllosphere microbial
Pyridoxal reductase
enzyme properties
