摘要
建立适合于临床应用的丙戊酸血浓度测定方法 ,进行临床药物浓度监测。方法 采用 Br MMC将丙戊酸转化为可通过紫外检测的酯 ,再经 RP-HPL C测定。结果 在 15～ 2 0 0μg/ml范围内 ,丙戊酸衍生物和内标的峰高比与浓度呈良好的线性关系 ,r=0 .9991。回收率平均为 10 1.2 %。应用于 3 4例患者的治疗药物监测 ,取得良好的临床效果。结论 方法准确、灵敏、选择性强、重现性好 ,操作简便快速 ,适于临床研究与应用。
Purpose To establish a RP HPLC method for analysis of valproic acid in human serum in clinic.Methods A RP HPLC method is applied with 4 bromomethyl 7 methoxycoumain as derivative reagent which transform valproic acid into an ester detectable with UV detector.Results The ratio of peak heights correlated linearly to concentration of valproic acid ranging from 15 to 200 μg/ml,r=0.9991.The mean recovery is 101.8%.The method has been applied in the therapeutic drug monitoring of 34 patients with epilepsy and satisfied clinical effects .Conclusion The method is sensitive,accurate,selective,simple and fast,and widely suitable for the clinical research and application.
出处
《华北煤炭医学院学报》
2000年第2期121-122,共2页
Journal of North China Coal Medical College
