摘要
目的:为了比较研究不同品牌基因工程。—:干扰素(IFNa_(2b)的抗肿瘤效应:方法:采用。—干扰素抑制多种肿瘤细胞生长的MTT法。结果:两种不同品牌的10000IU/ml,1000IU/ml和100IU/ml的IFNa_(2b)(干扰能和安达芬)对人脑胶质瘤细胞(SHG—44),人骨肉瘤细胞(HOS—8603),人白血病细胞(HL—60),红白血病细胞(K562)和淋巴瘤细胞(Raji)的生长均有不同程度的抑制作用。其抑制率分别为20％,10％和5％左右。结论:进口和国产的两种不同品牌IFNa_(2b)对上述五种肿瘤细胞株均有较明显的生长抑制效应,而且抑制百分率均相近。
Objective:To study the ami - tumor effect of two subtypes of the recombinant IFNa from different manufactories. Methods: MTT assay was applied to investigate the inhibition of various tumor cells growth by the two IFNa. Results: The growth of SHG-44, HOS-8603, HL-60, K562 and Raji cells were all inhibited by various IFNa2b at dose l0000IU/ml, l000lU/ml and 100IU/ml, with the according inhibitory rate about 20%, 10% and 5% respectively. Conclusions: Both the imported and domestic IFN a2b can produce remarkable inhibition effect on these five kinds of tumor cells, and the inhibitory rates are similar.
出处
《临床肿瘤学杂志》
CAS
2000年第3期158-160,共3页
Chinese Clinical Oncology
