摘要
目的：研究幽门螺杆菌（H.pylori）感染胃癌患者胃粘膜病变中抑癌基因p53、p16和关键性凋亡调节基因bcl-2蛋白的表达，进一步探讨H.pylori在胃癌发生、发展过程中作用的分子机制。方法：胃镜检查及外科手术中取40例胃癌患者的癌组织和癌旁2cm处组织各2块，石蜡包埋，切片HE染色作病理诊断及免疫组化检测p53、p16及bcl．2蛋白的表达。Hpylori阳性由快速尿素酶试验结合病理染色／14C-尿素呼气试验确定。结果：40例胃癌患者中，HPylori阳性23例，阴性17例。P53阳性表达率在H.pylori阳性及阴性胃癌组织中无显著差异（P＞005）。H．Pylori阳性组慢性胃炎或肠化组织中p16的阳性表达率及表达强度均显著低于H.pylori阴性组（表达率：P＜0.05；表达强度：P＜0．001和P＜0.01）。而H.pylori阳性组肠化组织中bcl-2的阳性表达率及表达强度均显著高于H.pylori阴性组（P＜0.05）。结论：在胃癌发生的早期即存在较明显的p16基因表达低下与bcl-2基因过度表达，并与H.pylori感染有一定关系。p53基因过度表达是胃癌发展过程中较晚期的事件，与H．pylori感染无明显相关性。
Background/Aims: To investigate the expression of tumor suppressive gene and regulatory geneof apoptosis in gastric mucosal lesions of gastric cancer patients with Helicobacter pylori (H. pylori) in-fection. Methods: 40 cases of gastric carcinoma patients underwent endoscopy and surgical resection,samples were taken from tumor site and paracancerous tissues. ABC immunohistochemical stainingwas used to detect the expression of p53, p16, bcl-2 proteins. H. pylori was determined by rapid ureasetest combined with pathological staining/14C-urea breath test. Results: There was no significant difference in the positive rate of p53 expression of gastric carcinoma between H. pylori-positive and H.pylori-negative group (P>0.05). The positive rate and staining intensity of p16 expression in patientswith chronic gastritis or intestinal metaplasia with H. Pylori-positive were significantly lower thanthose with H. pylori-negative (positive rate, P< 0.05; staining intensity, P< 0.001 and P< 0.01). In con-trast to p16, bcl-2 expression in patients with intestinal metaplasia with H. pylori-positive was signifi-cantly higher than that with H. pylori-negative (P<0.05). Conclusions: p16 or bcl-2 gene expressionalteration might play a role in the early development/promotion of gastric carcinoma, which is as-sociated with H. Pylori infection. It seems that H. pylori infection is not related to p53 gene alterationbecause p53 gene overexpression is a relatively late event in gastric tumorigenesis.
出处
《胃肠病学》
2000年第1期26-29,共4页
Chinese Journal of Gastroenterology
基金
江苏省教委自然科学基金!125FA9608
关键词
胃肿瘤
癌前状态
幽门螺杆菌
感染
基因
Stomach Neoplasms Precancerous Conditions Helicobacter pylori Helicobacter Infections Genes, p53 Genes, p16 Genes, bcl-2
