摘要
目的 通过观察胃癌及其癌前病变中细胞凋亡与细胞增殖间的关系，探讨细胞凋亡在胃癌发生中的作用．方法 利用脱氧核糖核酸末端转移酶介导的ｄ ＵＴＰ 缺口末端标记（ＴＵＮＥＬ） 技术及增殖细胞核抗原（ ＰＣＮＡ） 免疫组织化学染色对１０ 例正常胃粘膜、１６ 例萎缩性胃炎、３６ 例肠化生、２０ 例异型增生和５３ 例胃癌中的凋亡细胞、增殖细胞进行原位观察和比较．结果 萎缩性胃炎、肠化生、异型增生中凋亡细胞指数（１１－９ ％ ；１４－７ ％ ，８－０ ％ ） 均显著高于正常胃粘膜和 胃癌（３－５ ％ ，５－８ ％ ，ｔ ＝ ２－０５８ ～７－９０１ ，Ｐ＜ ０－０１ ～Ｐ ＜ ０－０５） ；异型增生、胃癌与肠化生相比，凋亡细胞明显减少、增殖细胞明显增多（ Ｐ＜ ０－０５） ；胃癌细胞增殖指数（４７－５ ％ ） 显著高于异型增生（３０－１ ％ ，Ｐ＜ ０－０１） ． 胃癌前病变及胃癌组织中的凋亡细胞指数与 增 殖细 胞指 数 呈显 著相 关（ ｒ ＝ ０－９６６ ， － ０－８９７ ，Ｐ＜ ０－０５） ．结论 胃粘膜癌变过程中不仅存在活跃的细胞增殖，而且存在细胞凋亡异常． 高增殖能力的细胞可能通过选择而占据优势，导致胃癌的发生．
AIM To investigate the role of apoptosis in the pathogenesis of gastric carcinoma by observation of the relationship between apoptosis and proliferation. METHODS Using terminal deoxynucleotidyl transferase_mediated nick end labelling (TUNEL) technique and PCNA immunohistochemical staining, we observed the apoptotic cells and proliferating cells in situ in 10 normal gastric mucosa, 16 chronic atrophic gastritis, 36 intestinal metaplasia, 20 gastric dysplasia and 53 gastric carcinomas. RESULTS The apoptotic indexes in atrophic gastritis, intestinal metaplasia and gastric dysplasia (11 9%, 14 7%, 8 0%) were significantly higher than those in normal mucosa and gastric carcinoma (3 5%, 5 8%, t =2 058-7 901, P <0 01-0 05). Comparison of intestinal metaplasia with gastric dysplasia and gastric carcinoma showed that apoptotic cells decreased and proliferating cells increased significantly. The PCNA index in gastric carcinoma (47 5%) was significantly higher than that in gastric dysplasia (30 1%, P <0 01). CONCLUSION Apoptosis may play a role in selection of clonal subpopulations with high growth potential resulting in malignant transformation. Abnormal regulation of apoptosis may play an important role in the pathogenesis of gastric carcinoma.
出处
《世界华人消化杂志》
CAS
1999年第8期649-651,共3页
World Chinese Journal of Digestology
基金
军队医药卫生九五重点课题资助项目
关键词
胃肿瘤
癌前状态
细胞凋亡
增殖细胞核抗原
stomach neoplasms
precancerous conditions
apoptosis
proliferative cell nuclear antigen
