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C3G在卵巢癌组织中的表达及其在SKOV3细胞增殖中的作用 被引量：3

Expression of C3G in epithelial ovarian cancer and its role in proliferation of SKOV3 cells
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摘要 目的探讨鸟苷酸交换因子C3G(Crk SH3-domain-binding guanine nucleotide-releasing factor)在卵巢癌组织中的表达及在卵巢癌SKOV3细胞增殖中的作用。方法采用Western blot检测比较40例卵巢癌、8例卵巢良性肿瘤及4例正常卵巢组织中C3G的表达水平。应用特异性siRNA下调SKOV3细胞中C3G的表达,通过MTT法和平板克隆实验检测细胞增殖,建立裸鼠皮下种植瘤模型观察移植瘤生长情况。结果卵巢癌组织中C3G的表达显著高于卵巢良性肿瘤及正常卵巢组织,其在卵巢癌、卵巢良性肿瘤分别是正常卵巢组织中的(1.832±0.200)(P<0.05)、(0.893±0.147)倍。siRNA干扰可使SKOV3细胞中内源性C3G的表达下调79.2%;与野生型细胞组、阴性对照组及空载体对照组相比,C3G干扰组细胞增殖变慢(P<0.05),克隆形成率下降(P<0.05),移植瘤的平均体积和质量显著下降(P<0.05)。结论鸟苷酸交换因子C3G在大多数上皮性卵巢癌中过表达,并促进卵巢癌细胞增殖。 Objective To determine the expression of guanine nueleotide exchange factor Crk SH3- domain-binding guanine nucleotide-releasing factor （C3G） in epithelial ovarian cancer tissues and its role in the proliferation of human ovarian cancer cell line SKOV3. Methods C3G was detected by Western blotting in the tissue samples of epithelial ovarian cancer （ n = 40）, benign ovarian tumors （ n = 8 ） and normal ovarian （n =4）, respectively. Endogenous C3G was knocked-down by siRNA in SKOV3 cells. Then, MTT assay and plate colony formation assay were used to evaluate the proliferation of cancer cells. Nude mice subcutaneous xenograft transplanted tumor model was established to assess tumor growth in vivo. Results The expression of C3G was significantly higher in epithelial ovarian cancer tissues than in benign ovarian tumors and normal ovari- an tissues. The expression of C3G was 1. 832 ±0. 200 times higher in epithelial ovarian cancer tissues（ P 〈 0. 05 ） and 0.893 ±0. 147 times higher in benign tumors when compared with normal ovarian tissues, respec- tively. Specific siRNA knocked down resulted in the expression of C3G decreased by 79.2% in SKOV3 cells, and significant decrease in cell proliferation and clone formation rate （P 〈 0. 05 ）. Nude mice xenograft trans- planted tumor by C3G siRNA transfected SKOV3 cells had average smaller volume and lower weight of sub- cutaneous tumors （P 〈0. 05）. Conclusion Nucleotide exchange factor C3G, over-expressed in most epithelial ovarian cancer tissues, facilitates the proliferation of ovarian cancer SKOV3 cells.
作者 罗淑娟 车亚玲 彭慧娟 张瑜 高一萌 刘斌 令狐华
机构地区 重庆医科大学附属第一医院妇产科 重庆医科大学基础医学院病理科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2013年第14期1471-1474,共4页 Journal of Third Military Medical University
基金 国家自然科学基金(81072120) 重庆市教委科学技术研究项目(KJ100303)~~
关键词 卵巢癌 鸟苷酸交换因子 C3G 增殖 human ovarian cancer guanine nucleotide exchange factor C3G proliferation
分类号 R394.2 [医药卫生—医学遗传学] R737.31 [医药卫生—肿瘤]
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参考文献18

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共引文献12

同被引文献29

  • 1侍立峰,梁志清.利用噬菌体肽库技术筛选卵巢特异的VEGFR3亲和肽[J].第三军医大学学报,2006,28(4):308-310. 被引量：7
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二级引证文献3

第三军医大学学报
2013年 第14期

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