摘要
目的分析星形胶质细胞上调基因-1(astrocyte elevated gene-1,AEG-1)可介导上皮-间质转化(epithelial-mesenchymal transition,EMT)调控头颈鳞癌侵袭转移的分子机制。方法采用慢病毒介导AEG-1的上调载体上调AEG-1在头颈鳞癌Tu686细胞中的表达,划痕愈合实验、Transwell侵袭小室实验检测其体外迁移侵袭能力的改变,同时Western blot检测EMT分子标志物E-cadherin及Vimentin的表达情况。结果 Tu686细胞中AEG-1表达上调后,细胞划痕愈合率增加,穿过Transwell聚碳酸酯膜的细胞明显增多,且Tu686细胞上皮细胞标志物E-cadherin的表达量显著下调,间质细胞标志物Vimen-tin表达显著上调。结论 AEG-1表达上调可显著提高头颈部鳞癌细胞的体外侵袭能力,且该侵袭能力的增强可能与AEG-1介导的EMT改变密切相关。
Objective To investigate the molecular mechanism of regulation of invasion and metastasis of squamous cell carcinoma in head and neck （SCCHN） via epithelial-mesenchymal transition （EMT） mediated by AEG- 1 in vitro. Methods Lentivirus-mediated AEG-1 overexpression vector was used for upregulating the expression of AEG-1 protein in SCCHN Tu686 cells. Wound healing assay, transwell chamber assay and Western blot were applied to examining the alteration of migration, invasion ability of Tu686 cells, and EMT molecular biomarkers （ E-cadherin and Vimentin ）. Results Overexpression of AEG-1 led to increase of both wound healing rate and number of cells penetrating transwell membrane, accompanied with down-regulated expression of epithelial molecule E-cadherin and up- regulated expression of mesenchymal molecule Vimentin. Conclusions AEG-1 overexpression can greatly enhance the migration and invasion ability of SCCHN cells in vitro, which may be associated with EMT changes mediated by AEG-1.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2013年第3期183-187,共5页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
国家自然科学基金项目(81071757
81272974
81202128)
教育部博士点基金项目(201001621)
湖南省博士研究生科研创新项目(CX2011B059)
湖南省科技厅重点项目(2010TP4012-1)
高等学校博士点专项科研基金新教师类(20120162120049)
中南大学青年助推基金(2012QNZT099)
关键词
头颈部肿瘤
癌
鳞状细胞
星形胶质细胞上调基因-1
上皮-间质转化
转移
Head and neck neoplasm
Carcinoma, squamous cell
Astrocyte elevated gene-1
Epithelial-mesenchy-mal transition
Metastasis
