摘要
作者对临床诊断视网膜母细胞瘤的患儿30例行前房穿刺、将房水用微型玻片离心沉淀仪沉淀,转速500r/min,离心5—10min后,以吖啶橙染色后行荧光显微镜检查,瘤细胞呈橙黄色荧光,易于辨认.30例检查结果均为阳性,其中15例行眼球摘除术,经病理学检查证实,另15例行血卟啉光动力治疗,保留眼球,随访3—5年,角膜穿刺口未见肿瘤转移.此法简单易行,阳性率高,为视网膜母细胞瘤的论断提供了一个较准确的方法.
The results of fluorescence microscopy on the cells of aqueous humor in 30 children with rctinoblastoma were presented . The aqueous aspirated through paracentesis of anterior chamber by using a small needle was put on a microslide and precipitated after centrifugation , stained with acridine orange and examined under fluorescence microscope . The tumor cells in the aqueous were easily identified by their orange - yellow color . Positive results were obtained in all patients (N=30). Among them the diagnosis of 15 patients was confirmed pathologically after surgery . In the other 15 patients , their eye balls were preserved and treated with HPD - PRT . No metastasis was found over the sites of paracentesis of the anterior chamber after 3 to 5 year's follow - up .The results indicate that fluorescence microscopic examination of the aqueous humor is a simple , safe and comparatively effective method with high positive rate for the diagnosis of retinoblastoma .
出处
《第四军医大学学报》
1991年第6期421-423,共3页
Journal of the Fourth Military Medical University
关键词
视网膜
母细胞瘤
眼肿瘤
眼房水
retinoblastoma
aqueous humor, acridine orangs
