摘要
以人早幼粒白血病细胞系(HL-60)为作用对象,对1,25(OH)_2D_3与小剂量化疗药物的体外合用效应进行了研究。将培养4d的细胞进行有关指标测定:NBT还原试验证实1,25(OH)_2D_3与小剂量高三尖杉酯碱(HH)或柔红霉素(DNR)有协同诱导分化效应;[~3H]TdR参入试验表明小剂最HH或DNR能弥补1,25(OH)_2D_3抑制增殖力较弱的不足;流式细胞分析(FCM)则提示经上述作用后的细胞中S期细胞显著减少,而大部分细胞停滞于G_0/G_1期。从而为诱导分化剂治疗白血病提供新的配伍方案。
The effects of 1, 25 (OH)2D3 in combination with low-dose chemotherapeutant on the human promyelocytic leukemic cells (HL-60) were investigated. In 4-day-cultured cells, 1, 25 (OH)2D3 and low-dose harringtonine (HH) or daunorubicine (DNR) had synergic effect in inducing differentiation of HL-60 cells, and HH or DNR significantly potentiated the effect of 1, 25 (OH)2D3 in inhibiting the cell proliferation, which were demonstrated by NBT reduction test and [3H]-TdR incorporation, respectively. Cell cycle analysis by flow cytometery revealed that the cells in S phase decreased remarkably and most cells stopped at G0/G1 phase. The results provide a new way for the inducers in treatment of leukemia.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第5期425-428,共4页
Academic Journal of Second Military Medical University
关键词
抗癌药
柔红霉素
HL-60细胞
antineoplastic agents
1, 25 (OH)2D3
harringtonine
daunorubicine
HL-60 cells
