摘要
本研究用二步液相层析法从小鼠颌下腺粗提物中纯化表皮生长因了(EGF),结果表明Bio-gel p-10分子筛和DEAE纤维素联合纯化EGF的效果较为理想。以纯化的EGF应用于动物的烧伤创面,发现有明显的促进创面愈合和减小疤痕收缩的作用。
The present article reports the method of preparation of epidermal growth factor (EGF) from mouse submaxillary glands by using a two-step liquid chromatography and the efficacy of EGF in promoting animal wound healing. It was found that combined purification of crude EGF is better with Bio-gel p-10 and DEAE-DE52 ion-exchange chromatographies and purified EGF has a marked enhancement of wound healing and reduction of cicatricial contracture in animals.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第2期132-135,共4页
Academic Journal of Second Military Medical University
关键词
表皮生长因子
创面愈合
烧伤
纯化
epidermal growth factor
purification
wound healing
mice
