摘要
用离体大鼠心脏冠状动脉前降支结扎10min后再灌注模型观察了小檗碱对再灌注心律失常的影响。冠状动脉结扎前10min给药,小檗碱呈剂量依赖性抗再灌注心律失常作用。小檗碱10^(-5)3.16×10^(-5)和10^(-4)mol/L使室颤发生率由对照组的100％降至90％、70％和40％(P<0.05)。心率也呈剂量依赖性降低,最高剂量小檗碱使心率从对照组的282±11次/min降至180±11次/min。小檗碱各剂量组冠状动脉流量均明显增加。小檗碱在有效剂量时经5Hz调搏其抗心律失常作用消失。小檗碱在再灌注前给药时无抗心律失常作用。α受体阻滞剂酚妥拉明使再灌注心律失常不发生,其效力远大于小檗碱。结果表明小檗碱的抗再灌注心律失常作用是由其负性频率作用所致。
Effect of-berberine on reperfusion arrhythmias was studied using a 10 rain left anterior descending coronary artery occlusion followed by reperfusion in the isolated rat heart. Berberine, when given 10min before coronary ligation, reduced the incidence of reperfusion arrhythmias in a dose-riependent manner. With 10-5, 3. 16×10-5 and 10-4 mol/L of berberine, ventricular fibrillation was reduced from its control incidence of 100% to 90%, 70% and 40% (P<0.05). Heart rate was also reduced in a dose-dependent manner, falling from its control value of 282±11 to 180±11 beats/min with the highest concentration of berberine. Coronary flow was increased in all berberine treated groups.With an antiarrhythmic dose of berberine (10-4 mol/L), hearts were paced (5 Hz) during ischemia period; under these conditions the anti-arrhythmic effect of berberine was lost. When berberine (3.16×10-5 and 10-4 mol/L) was administered just before reperfusion, no anti-arrhythmic effects were observed. The α-adrenoceptor antagonist phentolamine (2.6×10-5 mol/L) prevented reperfusion arrhythmias and is much more effective than berberine. These results suggest that the prophylatic effects of berberine on reperfusion arrhythmias in the isolated rat heart are due to its negative chronotropic properties.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1991年第1期1-4,共4页
Academic Journal of Second Military Medical University
关键词
小檗碱
心律失常
berberinp
arrhythmias
rats
