摘要
文章主要研究人参根总皂苷浓度和吸附温度对D101C大孔吸附树脂选择性吸附原人参二醇组皂苷(PPD)和原人参三醇组皂苷(PPT)影响。HPLC定量分析结果表明,人参根总皂苷浓度和吸附温度对PPD和PPT皂苷吸附影响显著,其中温度对PPD皂苷显示出较高选择性。当浓度为15 mg.mL-1人参根总皂苷溶液用D101C大孔吸附树脂于55℃吸附12 h,可吸附378.72 mg.g-1PPD皂苷和54.65 mg.g-1PPT皂苷,经35%和80%乙醇溶液依次解吸,可分离254.94 mg.g-1PPD皂苷,纯度94.62%。该方法操作简单,产品纯度较高,适用于工业化生产。
The influence of selective adsorption of protopanaxadiol and protopanaxatriol type Saponins was studied by using D101C macroporous adsorption resin, which focused on the tempera- ture and concentration of ginseng root Saponins. The results of HPLC analysis showed that the adsorp- tion capacities of PPD and PPT Saponins were significantly affected by the concentration of ginseng root Saponins and absorption temperature, absorption temperature displayed a highly selectivity for PPD Saponins among them. The solution of ginseng root Saponins （15 mg . mL-1） was adsorbed by using D101C macroporous adsorption resin at 55 ℃, for 12 h, the adsorption capacity of PPD Saponins was 378.72 mg. g-1, but that of PPT Saponins was only 54.65 mg. g-1. Then ginsenosides were succes- sively resolved by 35% and 80% ethanol at room temperature for 12 h to get 254.94 mg.g-1 of PPD Saponins （94.62%）. D101C macroporcous adsorption rein shows an excellent capacity to separate PPD Saponins, which is simple and suitable for industrial production, and the purity of PPD Saponins is high.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2013年第6期90-95,共6页
Journal of Northeast Agricultural University
基金
吉林市科技局项目(201222316)
关键词
D101C大孔吸附树脂
温度
选择性吸附
原人参二醇组皂苷
原人参三醇组皂苷
D101C macroporous adsorption resin
temperature
selective adsorption
protopan- axadiol type saponin
protopanaxatriol type saponin
