摘要
目的探讨丹蒲胶囊治疗慢性前列腺炎盆腔疼痛的分子机制。方法采用改良的实验性自身免疫性前列腺炎(EAP)法对大鼠造模,将造模后大鼠随机分为丹蒲胶囊高剂量组、中剂量组、低剂量组、前列泰组、模型组,给药8周后处死大鼠,取血清及前列腺组织,光镜下观察各组动物前列腺组织形态学变化,酶联免疫吸附试验(ELISA)检测血清及前列腺组织COX-2水平、实时逆转录聚合酶链反应(RT-PCR)法测定前列腺中COX-2-mRNA表达。结果 ELISA结果显示:模型组COX-2表达水平高于正常对照组,两组比较差异有统计学意义(P<0.01),高、中剂量组表达水平较模型组降低,差异均有统计学意义(P<0.01);RT-PCR结果显示:模型组COX-2-mRNA CT值较正常对照组降低,两者比较差异有统计学意义(P<0.01),高、中剂量的CT值较模型组比较升高,差异均有统计学意义(P<0.01)。结论 1.改良的EAP模型成功。2.丹蒲胶囊治疗CPPS的作用机制可能与其抑制COX-2表达有关。
Objective To investigate the molecular mechanism of Danpu Capsules in treating chronic prostatitis pelvic pain. Methods The rat model of improved experimental antoimmune prostatitis was established, then the rats were randomly divided into large-dosage,medium-dosage and low-dosage Danpu Capsules groups,Qianleitai group,model group. After 8-week medicine administration the rats were killed and the prostate tissue was taken for the observation of histomorphological changes of prostate with the light microscope in all groups. The COX-2 level of prostate tissue and serum was detected by enzyme-linked immunosorbent assay （ELISA） and the expression of COX-2-mRNA in prostate was determined by real-time reverse transcriptase polymerase chain reaction （RT-PCR）. Results The result of ELISA showed that the COX-2 level of the model group was higher significantly than that of the normal group （P〈0. 01 ）. After large and medium doses of medicine administration the COX-2 level of the Danpu Capsules group was decreased significantly （P〈0. 01 ）. The result of RT- PCR showed that the rat model of autoimmune prostatitis had higher expression of COX-2-mRNA,while in the large and medium doses of Danpu Capsules group this expression was inhibited（P〈0. 01）. Conclusion 1 The improved EAP rat model is successful. The mechanism of treating autoimmune prostatitis with Danpu Capsules maybe related to that Danpu Capsules can inhibit COX-2 expression.
出处
《云南中医学院学报》
2013年第3期16-20,共5页
Journal of Yunnan University of Traditional Chinese Medicine
基金
国家自然科学基金项目(NO:81250032/H2709)
北京市自然科学基金项目(NO:7112119
7112118)
