摘要
以构建RNA干扰表达体系,探索改良大豆品质方法为目的,根据GenBank中已知的大豆凝集素le2基因核酸保守序列(AY342212),设计相应引物,克隆le2基因核心保守序列,测序并与原序列比对同源性为99.77%。以质粒p3301-PFNZ-α'作为基础载体,通过亚克隆构建含有RNA干扰元件和bar基因的表达载体pCAMBIA3301-le2RNAi。通过农杆菌转化法将RNA干扰表达载体导入受体大豆吉农28,获得PCR阳性T1代植株5株,种子23粒。研究结果为大豆品质改良奠定了基础。
For the purpose of constructing RNAi expressed vector, and discussing the method to improve soybean quality, ac- cording to known conserved sequence（AY342212） of soybean agglutinin le2 gene in GenBank, right primer was designed and the conserved sequence was cloned. And the homology was 99.77% contrast to the original sequence. Herein, using P3301-PF- NA-α＇as the basic vector, the expression vector pCAMBIA3301-le2RNAi was constructed which contained RNA interference vector and bar gene through subcolone. The RNA interference expression vector was then transformed into cotyledon nodes of soybean Jinong 28 by Agrobacterium-mediated method. 5 positive transgenic plants in T1 generation were confirmed by PCR de- tection and 23 seeds were harvested from T1 transgenic plants. The results provided a basis for soybean quality improvement.
出处
《大豆科学》
CAS
CSCD
北大核心
2013年第3期306-309,共4页
Soybean Science
