摘要
采用20个ISSR标记对来自福建湄洲岛的40份烟豆(G.tabacina)材料进行了遗传多样性分析以促进烟豆资源的保护开发和利用。结果表明:20个ISSR引物对40份烟豆基因组DNA均可进行有效扩增,共扩增出240条谱带,其中多态性谱带229条,多态性比率为95.4%;20个ISSR引物的多态性信息含量为0.03～0.37,平均为0.27;40份烟豆材料之间的Dice相似系数为0.335 6～0.863 9,平均为0.675 3,说明湄洲岛烟豆材料遗传变异较丰富;聚类分析将40份烟豆材料划分为4个类群,每个类群都包括来源于不同采集地点的材料,说明不同采集地点之间的材料存在基因交流。
In this study, the genetic diversity of 40 accessions of G. tabacina, collected from Meizhou Island of Fujian province were analyzed using 20 ISSR markers to promote the protection,exploration and utilization of G. tabacina. A total of 240 bands were amplified,of which 229 bands, accounting for 95.4%, were polymorphic. The average polymorphism information content （PIC） of 20 ISSR primers was 0.27, ranging from 0.03 to 0.37. The Dice similarity coefficient of the tested accessions ranged from 0.335 6 to 0.863 9, and averaged 0.675 3. The tested G. tabacina were clustered into four groups by using the unweight- ed pair-group method with arithmetic averaging（ UPGMA）, and each group contained one or more accessions from different col- lection sites. Results suggest there is rich of genetic diversity among the accessions of G. tabacina,and existing gene exchange among G. tabacina accessions from different collection sites.
出处
《大豆科学》
CAS
CSCD
北大核心
2013年第3期286-290,共5页
Soybean Science
基金
公益性行业(农业)科研专项(201003021)
农业部野生资源保护与利用项目
吉林省现代农作物种业发展专项资金项目
