摘要
目的:从分子生物学角度探讨电针督脉"长强"和"百会"穴对自闭症的作用机制。方法:采用Wistar孕鼠腹腔注射丙戊酸钠(VPA)的方法建立自闭症大鼠模型,选取自闭症模型仔鼠40只,随机分为模型组、非穴组、电针长强组、电针百会组,另外选取正常生产的仔鼠10只作为空白组。电针长强组:针刺大鼠"后海"穴(相当于人体的"长强"穴),连接电针仪,施以连续波,频率2Hz,时间20min,每日1次,连续治疗20d;电针百会组:选取"百会"穴,非穴组选取大鼠右侧胁下固定非经非穴点,电针操作同电针长强组;空白组和模型组在同等条件下饲养,不予任何干预。应用Morris水迷宫观察各组大鼠逃避潜伏期和原平台象限游泳路程/总路程的比值;应用免疫组化技术观察各组大鼠海马CA1区突出后致密物(PSD-95)蛋白表达情况。结果:与空白组比较,模型组和非穴组的逃避潜伏期明显延长(均P<0.05),原平台象限游泳路程/总路程的比值明显降低(均P<0.05),PSD-95蛋白表达明显降低(均P<0.05);与模型组比较,电针长强组和电针百会组的逃避潜伏期明显降低(P<0.05),原平台象限游泳路程/总路程的比值升高(均P<0.05),PSD-95蛋白表达明显升高(P<0.05);电针百会组与电针长强组比较,逃避潜伏期、原平台象限游泳路程/总路程和PSD-95蛋白表达均无明显差异(P>0.05)。结论:单独电针长强穴或百会穴均可改善自闭症模型大鼠的学习和记忆能力,且二者间无显著性差异。其作用机制可能与调控PSD-95蛋白的表达相关。
Objective To explore the effect mechanism of electroacupuncture （EA） at Changqiang （GV 1） or Baihui （GV 20） on autism based on molecular biology. Methods The autism model was established by intraperito- neal injection of sodium valproate （VPA） in Wistar pregnant rats. Forty young rats with autism were selected and randomly divided into a model group, a non-acupoint group, an electroacupuncture at ＂Changqiang＂ （GV 1） （EAGV 1 for short） group and an electroacupuncture at ＂Baihui＂ （GV 20） （EAGV 20 for short） group. Another 10 normal young rats were selected as a blank group. In the EAGV 1 group, acupuncture was applied at Houhai ~as Chang- qiang （GV 1）], then EA apparatus was connected with continuous wave, 2 Hz, 20 rain, once a day for consecu- tive 20 days. The same EA manipulation as EAGV 1 group was used in the EAGV 20 group where ＂Baihui＂ （GV 20） was selected and non-acupoint group where non-acupoint in the right rib was selected. Blank group and model group were reared under the same conditions without any intervention. The escape latency and the ratio of swimming distance in platform quadrant to total swimming distance in each group were observed by using Morris water maze, and the PSD-95 protein expression in hippocampal CA 1 was measured by immunohistochemical tech- niques. Results Compared with the blank group, the escape latency in the model group and the non-acupoint group lengthened （both P〈0.05）, the ratio of swimming distance in platform quadrant to total swimming distance was decreased （both P〈0.05）, the PSD-95 protein expression was decreased （P〈0.05）. Compared with the model group, the escape latency in the EAGV 1 group and the EAGV 20 group were decreased （both P〈0. 05）, the ratio of swimming distance in platform quadrant to total swimming distance was increased, the PSI）-95 proteinexpression was increased （both P〈0.05）. But the escape latency, the ratio of swimming distance in platform quadrant to total swimming distance and the PSD-95 protein expression had no significant difference between EAGV 1 group and EAGV 20 group （P2〉0.05） Conclusion Electroacupuncture at Changqiang （GV 1） or Baihui （GV 20） can respectively improve learning and memory ability of rats with autism, which has no significant differe- nce and the mechanism of action may be related to regulation of the PSD-95 protein expression.
出处
《中国针灸》
CAS
CSCD
北大核心
2013年第7期627-631,共5页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金资助项目:81202764
福建省教育厅资助课题:JK 2012022
福建省科技厅资助课题:2012Y0045
关键词
自闭症
电针
督脉
学习记忆能力
突出后致密物
autism
electroacupuncture
Governor Vessel
learning and memory ability
PSD-95
