摘要
目的:制作一种新的可控制狭窄程度的大鼠颈动脉狭窄模型,观察其早期认知功能改变。方法:采用双侧颈总动脉不完全阻断法建立颈动脉狭窄模型,并在DSA下行血管造影进行验证,60只大鼠随机分为正常对照组,假手术对照组模型组、(中度狭窄组及重度狭窄组)。分别进行水迷宫测试及测定P300以评价其认知功能。结果:可建立稳定的不同程度的颈动脉狭窄。处理段动脉经计算机图形处理系统计算后显示轻、中、重不同程度狭窄;狭窄组大鼠Morris水迷宫隐匿平台逃避潜伏期较假手术组明显延长(P<0.01),重度狭窄组大鼠较中度狭窄组大鼠延长更明显(P<0.01);空间探索实验中对照组平台象限游泳距离占总游泳距离为(54.97±7.51)%,中度狭窄组为(40.17±7.83)%,重度狭窄组为(29.50±4.19)%。狭窄组大鼠平台象限游泳距离占总游泳距离的百分比明显低于对照组(P<0.01),重度狭窄组大鼠较中度狭窄组更低。P300测定正常对照组潜伏期为(275.14±12.48)ms,中度狭窄组为(340.43±8.48)ms,重度狭窄组为(357.00±4.32)ms。狭窄组大鼠潜伏期较假手术对照组明显延长(P<0.01),重度狭窄组大鼠较中度狭窄组大鼠延长更明显(P<0.01)。2周海马苏木精-伊红染色狭窄组大鼠CA1区锥体细胞排列紊乱,细胞体积和胞核均浓缩、深染,结构不清。结论:不完全阻断颈?
AIM: To establish a new kind of controlled carotid stenosis model in rat, and observe the changes of cognitive function at early time. METHODS:A rat model of carotid stenosis was established by means of incompletely bilateral carotid occlusion, and testified by digital subtraction angiography.Sixty Wistar rats were randomly divided into control group,sham operation group and model group(moderate stenosis group and severe stenosis group). The values of P300 and the results of experiments in water maze test were recorded respectively, which was used to evaluate the cognitive function. RESULTS:The different level of carotid stenosis was established stably,and evaluated with the system of computer graphy, including mild, moderate and severe. The escape latency of Morris water maze was longer in stenosis group than in sham operation group(P< 0.01), and was much longer in severe stenosis group than in moderate stenosis group(P< 0.01).In spatial probe test, the percentage of swimming distance in the former platform was (54.97±7.51)%,(40.17±7.83)%and(29.50±4.19)%in control group, moderate and severe stenosis group respectively. The percentage of swimming distance in the former platform in stenosis group was significantly lower than that in control group(P< 0.01), which in severe stenosis group was much lower than that in moderate stenosis group. The latency tested by P300 was(275.14±12.48) ms,(340.43±8.48) ms and(357.00 ±4.32) ms in normal control group, moderate and severe stenosis group respectively. The latency was longer in stenosis group than in sham operation control group(P< 0.01), and was much longer in severe stenosis group than in moderate stenosis group(P< 0.01). With hematoxylin and eosin(HE) staining for two weeks, the arrangement of pyramidal cell in CA1 was irregular in stenosis group, and all cell volum and nuclei were condensed, deeply stained and constructed unclearly. CONCLUSION: Incomplete carotid occlusion can establish various degrees of carotid stenosis model, including mild, moderate and severe. Cognitive function in animal becomes more severe with the development of stenosis. This kind of model is an ideal model to study carotid stenosis and vascular dementia.
出处
《中国临床康复》
CSCD
2004年第28期6052-6054,i001,共4页
Chinese Journal of Clinical Rehabilitation
