摘要
目的:观察风湿宁胶囊(FSN)对胶原诱导性关节炎(CIA)大鼠滑膜细胞的促凋亡作用并探讨其作用机制。方法:制备CIA大鼠模型,将大鼠随机分为正常组、CIA模型组、雷公藤多苷组、FSN低、中、高剂量组。采用ELISA法检测滑膜细胞色素C(Cytochrome C)含量,免疫组化法检测滑膜中B细胞淋巴瘤-2(Bcl-2)的表达,Western Blot法分析FSN对滑膜含半胱氨酸的天冬氨酸蛋白水解酶-3(Caspase-3)蛋白表达的影响。结果:FSN能够明显升高Cytochrome C水平(P<0.05),下调Bcl-2蛋白的阳性表达,促进Caspase-3蛋白的表达(P<0.05)。结论:风湿宁胶囊能诱导CIA大鼠滑膜细胞凋亡,其促凋亡机制可能与抑制Bcl-2表达,促进Cytochrome C释放入胞浆,激活Caspase-3介导的细胞凋亡线粒体途径有关。
Objective： To investigate the pro-apoptosis effect and mechanism of Fengshining Capsule （FSN） on collagen-induced arthritis （CIA） in rats synoviocyte. Methods： Rats were divided into normal comparison group, CIA model group, tripterygium glycosides group, FSN low dose group, FSN middle dose group and FSN high dose group. The content of Cytochrome C in synovium of rats was detected by ELISA assay, and its expression of Bcl-2 in syuoviocyte was detected by immunohistochemical assay. Caspase-3 protein expression was measured by Western Blot. Results： FSN significantly increased the levels of Cytochrome C （P〈0.05）, and reduced the expression of Bcl-2 protein. Meanwhile, the expression of Caspase-3 was promoted too （P〈0.05）. Conclusion： Fengshining Capsule might induce synoviocyte apoptosis in CIA rats. Its mechanism may be related to inhibiting the expression of Bcl-2, releasing Cytochrome C into the cytoplasm and activating mitochondrial pathway of apoptosis-related by Caspase-3 mediated.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第7期1985-1988,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家科技重大专项(No.2010ZX09102-210)
山西省科技产业化环境建设项目(No.2012061014-3)~~
