摘要
为弄清噻二唑苯基脲(TDZ)对粉蕉离体茎尖芽诱导及增殖的效果,采用外植体诱导芽增殖继代培养后接种的方法,对其进行了试验。结果表明:MS+TDZ 0.2～2.0mg/L处理比对照吸芽褐化率低20～33百分点,芽月增殖率达7.1～18.9倍,增殖周期短15～20d;其中MS+TDZ 0.2～0.5mg/L比对照褐化率低30～33百分点,芽月增殖率高7.1～8.1倍,增殖周期短15～20d。MS+TDZ 0.2～0.5mg/L在降低粉蕉吸芽褐化率和褐化程度方面具有显著效果,极显著提高粉蕉芽月增殖率,同时缩短增殖周期。
In order to explore the effects of TDZ on in vitro stem tip induction and proliferation of dwarf banana,an experiment was conducted by inoculating stem tips after induction and proliferation.The results showed that the browning rate of MS＋TDZ 0.2~2.0 mg/L was lower than the control by 20~33 percents.The monthly shoot proliferation rate was 7.1~18.9 times of the control.The proliferation cycle was shortened by 15~20 days;The browning rate of the treatment MS＋TDZ 0.2~0.5 mg/L was lower than the control by 30~33 percents.The monthly shoot proliferation rate was 7.1~8.1 times of the control,the proliferation cycle was shortened by 15~20 days.MS＋TDZ 0.2~0.5 mg/L had significant effect in reducing the dwarf banana bud browning rate and browning degree,significantly increased the proliferation rate and shortened the proliferation cycle.
出处
《贵州农业科学》
CAS
北大核心
2013年第6期30-32,共3页
Guizhou Agricultural Sciences
基金
贵州省院所创能科技计划项目"贵州生物质能源植物研发创新能力建设"[黔科合院所创能(2010)4007]
关键词
TDZ
粉蕉
组织培养
离体茎尖
芽诱导
TDZ
dwarf banana
tissue culture
in vitro shoot-tip
bud induction
