摘要
选取‘改良蒜’、‘金堂早蒜’、‘苍山蒜’和‘欧引01’等4个不同生态型的大蒜品种,在含百草枯浓度分别为0.2%、0.4%、0.6%、0.8%、1.0%、1.5%的培养基上采用直接接种和逐级培养法,选择抗百草枯愈伤组织细胞系。结果表明:4个品种在低浓度(﹤0.4%)胁迫下都有一定的抗性,经逐级抗百草枯选择后的愈伤组织,其抗性能力有明显提高。在含有百草枯浓度0.6%培养基上,4个品种愈伤组织的成活率为7.8%～20.6%,且发育良好;在浓度0.8%和1.0%的百草枯培养基上也有部分愈伤组织成活。抗性稳定性检验表明,大蒜抗百草枯能力在一定时间内能够保持下去。
Four different ecotypic garlic cultivars 'modified garlic ',' Jintang early garlic ',' Cangshan 'and 'Europe 01 'were chosen to select for the resistant paraquat callus,in the medium including paraquat concentrations 0.2%,0.4%,0.6%,0.8%,1.0% and 1.5%,respectively.The results showed that the four cultivars had some resistance under the low concentration stress (﹤ 0.4%),and the resistance of the callus were enhanced by step-to-step culturing method.The survival rates of the callus were from 7.8% to 20.6% in the medium including containing 0.6% paraquat.Some callus was also survived in the medium containing 0.8% and 1.0% paraquat.Stability test showed the ability of resistant paraquat could keep for a long time.
出处
《中国农学通报》
CSCD
北大核心
2011年第2期104-108,共5页
Chinese Agricultural Science Bulletin
基金
中德政府间合作项目"马铃薯
大蒜体细胞无性系变异筛选利用研究"(CH-01/04)
关键词
大蒜
愈伤组织
变异系
抗百草枯选择
garlic
callus
variation line
resistant paraquat selection
