摘要
通过对NK58S和NK58F这一对光敏核不育水稻等位突变系的AFLP分析,比较了AFLP,RAPD及RFLP检测DNA多态性的相对效率。结果表明,这三种分子标记的DNA多态性检出效率依次为AFLP>RAPD>RFLP;找出了水稻AFLP分析的最适反应条件;通过AFLP和集群混合分析(Bulkedsegregatinganalysis,BSA),筛选出了一批与水稻光敏核不育(PGMS)基因连锁的多态性AFLP产物,已完成了对4个多态性AFLP产物的克隆,Southern杂交证明其中2个为单拷贝顺序,另外2个为低拷贝顺序。对上述三种分子标记各自的优缺点及它们在DNA多态性检测中的适用之处进行了分析探讨。
The reaction conditions for rice AFLP assay were optimized.The relative efficiencies for polymorphism detection of RFLP,RAPD and AFLP were compared through the analysis between a pair of PGMS allelic mutant lines(NK58S and NK58F).Results indicated that the efficiency for polymorphism detection in rice is in the order of AFLP>RAPD>RFLP,and also indicated that AFLP is a powerful DNA molecular marker technique for polymorphism detection,especially in the cases of extremely low polymorphism,such as isogeneic lines and allelic mutant lines.The advantages and disadvantages of these three molecular marker systems were discussed.Using AFLP in conjunction with bulked segregating analysis,5106 AFLP loci were screened and 9 of them showed polymorphism between NK58S and NK58F,4 of the polymorphic AFLP products were cloned,Southern bloting analysis showed that two of them were single copy sequences while the other two were low copy sequences in rice genome.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第1期91-95,共5页
Chinese Journal of Biotechnology
基金
国家高技术研究发展计划项目!( 86 3 HB 0 1 0 2 0 2 )
洛克菲勒水稻生物技术项目!(RF96 0 0 1# 42 4)资助
