摘要
目的探讨原发性食管鳞癌(ESCC)组织中mir-183的表达及其与患者临床病理特征、基因表达及预后之间的关系。方法检测83例ESCC组织及癌旁正常组织中mir-183以及基因的表达状况,并对患者的临床病理特征及预后进行分析。结果83例标本中mi r-183表达上调51例(61.4%),mi r-183表达的上调与ESCC患者的淋巴结转移、拓扑异构酶Ⅱ(TopoⅡ)表达及不良预后有关(均P<0.05)。结论 mir-183的表达上调与ESCC的淋巴结转移及TopoⅡ的表达状况有关,并提示预后不良。
Objective To investigate the expression of microRNA-183 （mir-183） in esophageal squamous-cell carcino-ma（ESCC）and its correlation with disease prognosis. Methods The expression of mir-183 was detected by real-time RT-PCR and immunohistochemistry in 83 ESCC tissue specimens and corresponding pericancerous tissues. Results Among 83 ESCC samples mir-183 was significantly up-regulated （2~6411-fold） in 51 cases compared to the matching pericancerous tissues. Up-regulation of mir-183 expression was correlated with lymph node metastasis, expression of ToPoⅡ and poor prognosis in ESCC patients （al P〈0.05）. Conclusion Up-regulation of mir-183 expression may indicate the poor prognosis of patients with esophageal squamous cel carcinoma.
出处
《浙江医学》
CAS
2013年第10期849-851,855,共4页
Zhejiang Medical Journal
基金
卫生部科研基金资助项目(WKJ2010-2-004)
浙江省自然科学基金资助项目(Y2080749
Y2091110)
浙江省科技计划基金资助项目(2009C33143)
