摘要
目的对模拟流感自然感染方式建立的小鼠流感模型进行初步探索。方法分别选用H1N1FM1和H1N1PR8两种病毒采用气雾攻击的方法感染ICR小鼠，每日称小鼠体质量，肉眼观察小鼠状态，于感染后5d、12d、21d处死小鼠，取肺脏称其湿重，分别做肺脏的病毒测定及病理观察。结果感染两种毒株的ICR小鼠在感染早中期临床症状明显，体质量严重下降，肺指数明显升高，病理变化明显，出现了典型肺水肿，肺间质炎及肺充血等病变，并在感染早期小鼠肺脏中检测到流感病毒。感染晚期小鼠症状逐渐减轻，未检测到病毒。结论通过气雾攻击的方法来建立流感病毒气溶胶感染小鼠模型是可行的。
Objective Preliminary study on the model of influenza which was established by simulation of human natural infection in mouse. Methods ICR mice were infected with H 1N 1 FM 1 and H1N1 PR8 virus by aerosol inhalation. The symptoms and body weight of mice were observed everyday. At 5, 12, 21 day after infection,the mice were sacrificed. The lungs of mice were weighed, then were done virus assay and pathological observation. Results The clinical symptoms of infection mice were obvious, the body weight lost seriously, lung index increased and pathological changes signifi- cantly in the early mid-term, for example the typical pulmonary edema, pulmonary interstitial disease, inflammation and pulmonary congestion appeared in the lung of mice. The influenza virus also can be detected in the early stage. The symptoms of mice getting better, the virus were not detected in the late. Conclusions The established infection mouse model of the influenza virus aerosol by aerosol inhalation is feasible.
出处
《实验动物与比较医学》
CAS
2013年第3期180-184,共5页
Laboratory Animal and Comparative Medicine
基金
上海市科委资助项目（12140903100）
