摘要
目的 从分子生物学角度探讨内皮素 (ET)、一氧化氮 (NO)与动脉硬化闭塞症 (ASO)的相互关系。方法 选择 96例 ASO患者和 86只模型 ,利用放射免疫、Griss、光电比色和逆转录 PCR方法检测血浆 ET、NO和血管一氧化氮合酶 (NOS)活性、ET m RNA和NOS m RNA表达情况。结果 ASO患者和模型存在高 ET血症和低 NO血症 ,ASO病变血管存在低 NOS活性、低 NOSm RNA表达和高 ET m RNA表达状态 ,并同 ASO严重程度密切相关。结论 高 ET血症、低 NO血症、低 NOS活性、低 NOS m RNA表达和高 ETm RNA表达状态同 ASO的发生发展密切相关 ,ET、NO、NOS活性、NOS m RNA和 ET m RNA表达的检测有助于 ASO发病机制的探讨 ,有助于病情严重程度和治疗效果的判断。
The relationship and its clinical significances between endothelins(ET), nitric oxide(NO), and atherosclerosis obliterans(ASO) were investigated.Methods 96 ASO patients and 56 ASO rabbit models were selected with their plasma ET, NO, NOS activity, and ET mRNA, NOS mRNA expression determined by radioimmunoassy, colorimetry and RT PCR methods.Results The ASO patients and models had high plasma ET levels and low plasma NO levels related with the severity of ASO, but the ET mRNA expressions were increased gradually, the NOS mRNA expressions and the NOS activity decreased gradually. All the above changes gradually returned to mormal after treated by PGE 1.Conclusions The abnormalitis of ET, NO, NOS activity, ET mRNA, NOS mRNA expressions closely relate with ASO severity and play an important role in the ASO pathogenesis, and can be used to judge the severity of the condition and the effectiveness of treatments.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2000年第5期260-262,共3页
Chinese Journal of Gerontology
基金
中国博士后基金
