摘要
蜡样芽孢杆菌(Bacillus cereus)是一种在环境中广泛存在,极易污染食品及食品原料的细菌,也是重要的食源性条件致病菌。目前,国内检测食品中蜡样芽孢杆菌仍采用传统的方法,其操作繁琐、特异性低。为提高检测效率,开发出一种蜡样芽孢杆菌显色培养基(HKMCB),与国外OXOID同类产品蜡样芽孢杆菌显色培养基(OXCB)及传统培养基MYP在特异性、选择性及灵敏度方面进行了比较和初步评价。结果显示,HKMCB显色培养基均具有较好的特异性,优于传统培养基MYP。HKMCB及OXCB显色培养基均具有较好的选择性,对非目标菌抑制率比传统培养基MYP高50%。8株蜡样芽孢杆菌在3种培养基上的生长率均大于0.8。经统计分析,试验的3种培养基生长率差异不显著(P>0.05)。结论:利用显色培养基检测蜡样芽孢杆菌,较常规的生化检测方法具有较高的特异性和选择性,检测效果良好。
Bacillus cereus is an important food-borne opportunistic pathogen which widely exists in environment and food. Because of the deficiencies of complicated operation and low specificity in the general method of the isolation and identification of B.cereus, a new chromogenic medium （HKMCB） was designed in this assay. Through detecting the reference strains, the specificity, selectivity and sensitivity of HKMCB were studied and compared with OXCB and MYP. The results showed that the specificity of HKMCB were better than that of MYP. The selectivity of HKMCB was same as that of OXCB, and 50% higher than that of MYP for non target bacteria inhibition. The productivity of 8 strains on three culture were all above 0.8, After statistical analysis of these results, there are no significant difference among three culture media （P〉0.05） in this study. In conclusion, B. cereus chromogenic medium has a good effect in detecting B. cereus because it has high specificity and selectivity.
出处
《食品科技》
CAS
北大核心
2013年第6期313-317,共5页
Food Science and Technology
基金
广东省重大科技专项(2012A080203014)
广州市发改委生物产业项目(2012B031000019)
