摘要
目的:探讨山豆根颗粒及其饮片的抗炎作用机制。方法:采用角叉菜胶致小鼠足肿胀、棉球致肉芽肿实验模型,以地塞米松为阳性对照,观察山豆根颗粒及其饮片高、中、低剂量(生药7.0,3.5,1.75 g.kg-1)对角叉菜胶致小鼠足肿胀的影响,并测定小鼠角叉菜胶炎性渗出液中前列腺素E2(PGE2)含量,棉球致炎小鼠肾上腺质量及测其维生素C(Vit C)的含量;采用内毒素(LPS)造成小鼠急性炎症模型,观察山豆根颗粒及其饮片对小鼠血清一氧化氮合酶(NOS)、一氧化氮(NO)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)等含量的影响。结果:与模型对照组比较,山豆根颗粒及其饮片各剂量均能显著抑制小鼠足肿胀度(P<0.05或P<0.01),山豆根颗粒及其饮片高、中剂量组能抑制小鼠肉芽质量(P<0.05或P<0.01);山豆根颗粒及其饮片组高剂量组能降低小鼠肾上腺中Vit C、肿胀足PGE2及血清MDA,NO,NOS,TNF-α,IL-6等的含量(P<0.05或P<0.01)。结论:山豆根颗粒及其饮片抗炎作用机制可能与与其清除氧自由基,抑制细胞膜脂质过氧化反应,减少炎症因子释放,抑制细胞因子的表达等途径有关。
Objective： To explore the anti-inflammatory effect and related mechanism of Sophora tonkinensis granula and its herbal piece.Method： The effect of Sophora tonkinensis granula and its herbal piece（7.0,3.5,1.75 g.kg-1） on carrageenan foot swelling in mice was determined,and the content of proateglandin E2（PGE2） in inflammatory exudate was measured.Tampons induced inflammation in mice was employed,and adrenal weight and vitamin C（Vit C） content were determined.Acute inflammation model was established in mice with lipopolysaccharide（LPS）,and the influence of Sophora tonkinensis granula and its herbal piece on mice serum nitric oxide synthase（NOS）,nitrogen monoxide（NO）,maleic dialdehyde（MDA）,tumour necrosis factor-α（TNF-α） and interleukin-6（IL-6） were determined.Result： Compared with model control group,Sophora tonkinensis granula and its herbal piece in each dose groups all could significantly inhibit foot swelling degree in mice（P ＆lt; 0.05 or P ＆lt; 0.01）.Sophora tonkinensis granula and its herbal piece high,middle dose group can inhibit mice granulation weight（P ＆lt; 0.05 or P ＆lt; 0.01）.Sophora tonkinensis granula and its herbal piece of high dose group could reduce adrenal Vit C,inflammation foot PGE2 and serum MDA,NO,NOS,TNF-α,IL-6 content（P ＆lt; 0.05 or P ＆lt; 0.01）.Conclusion： The anti-inflammatory mechanism of Sophora tonkinensis granula and its herbal piece may be related to its ability of scavenging oxygen free radicals,inhibiting lipid peroxidation reaction,reducing the release of inflammatory cytokines,inhibiting cytokine expression and so on.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期265-269,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技厅科技攻关课题(桂科攻0815005-1-9)
关键词
山豆根颗粒及其饮片
抗炎作用
机制
sophora tonkinensis granula and its herbal piece
anti-inflammatory effect
mechanism
