摘要
目的:探讨冠心舒通胶囊及其各单味药对体外培养乳鼠心肌细胞和主动脉平滑肌细胞缺氧/复氧(hypoxia/reoxygenation,H/R)损伤以及细胞增殖的影响。方法:用MTT比色法,考察广枣提取物、丹参提取物、天竺黄水提物、天竺黄醇提物、冰片和胶囊内容物(0.01,0.05,0.10,0.20μg.L-1)对血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞和主动脉平滑肌细胞增殖的影响。取原代培养的乳鼠心肌细胞和主动脉平滑肌细胞,建立H/R细胞损伤模型,实验分为正常细胞对照组(NC)、模型组(H/R)、以上各给药组(0.01,0.05,0.10μg.L-1),给药24 h后测定细胞内超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)、一氧化氮合酶(NOS)、一氧化氮(NO)的水平。结果:与正常组比较,模型组细胞存活率明显降低,LDH漏出量增加、MDA含量增加、NO含量减少,NOS活性降低,SOD活性降低;与模型组比较,给药组可明显提高细胞存活率,提高细胞内SOD活性,增加NO含量,减少LDH漏出量和MDA含量,提高NOS活性。结论:冠心舒通胶囊及其各单味药对H/R损伤的心肌细胞和主动脉平滑肌细胞具有保护作用,其机制与抗脂质过氧化和舒张血管以及抑制细胞增殖有关。
Objective： To explore the protective effects of Guanxin Shutong capsules（GSC） and its single herbs on hypoxia/reoxygenation（H/R） injury and proliferaion in primary cultured myocardial cells and aortal smooth muscle cells.Method： The protective effects of six groups of drugs with four carnitine concentrations（0.01,0.05,0.10,0.20 μg.L-1）,including Choerospondia axillaris extracts of Salvia miltiorrhizae,water extracts of Bambusa texeilis,alcohol-extract B.texeilis,Borneol and GSC,on the proliferation of myocardial cells and aortal smooth muscle cells induced by Angiotension Ⅱ were detected with MTT colorimetric method.（H/R） cell model was established by cultured primary myocardial cells and aortal smooth muscle cells of neonatal rats.The cells were divided into normal control group,H/R group and administration group（0.01,0.05,0.10 μg.L-1）,after 24 hours,lactate dehydrogenase（LDH）,malondialchehyche（MDA） and superoxide dismutase（SOD）,nitric oxide（NO） and nitric oxide synthase（NOS） were measured.Result： Compared with the normal control group,the survival rate of cells,SOD,NO and NOS in H/R group were obviously decreased,while LDH,MDA were obviously increased.Compared with the H/R group,the survival rate of cells,SOD,NO and NOS in administration group were obviously increased,while LDH,MDA were obviously decreased.Conclusion： GSC and its single herbs can protect myocardial cells and aortal smooth muscle cells injury induced by H/R,and the mechanism may be related with the effects on anti-lipid peroxidation and vasodilation of blood vessels and inhibition of cell proliferation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期226-230,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十二五"重大新药创制科技重大专项(2011ZX09401-308-6)
关键词
冠心舒通胶囊
单味药
心肌细胞
主动脉平滑肌细胞
保护作用
Guanxin Shutong capsules
single herbs
aortal myocardial cells
smooth muscle cells
protective effect
