摘要
目的:研究3'-大豆苷元磺酸钠对大鼠脑缺血再灌注损伤后视网膜作用的影响。方法:采用Longa方法制备大脑中动脉脑缺血再灌注损伤模型,一次性给予不同剂量3'-大豆苷元磺酸钠(1.0,2.0 mg.kg-1)舌下静脉注射。观察给药后脑梗死范围和脑组织含水量的影响。用夹闭大鼠一侧颈总动脉制备大鼠视网膜缺血模型。夹闭颈总动脉前,给药组分别舌下静脉注射不同剂量3'-大豆苷元磺酸钠(1.0,2.0 mg.kg-1)。观察对夹闭大鼠颈总动脉前及灌注后1 h眼底荧光血管造影和视网膜电流图的影响。结果:与模型组相比,1.0,2.0 mg.kg-1的3'-大豆苷元磺酸钠能显著降低脑组织含水量(P<0.05);与模型组比,1.0,2.0 mg.kg-1的3'-大豆苷元磺酸钠能使视网膜电流图振幅显著增加(3.72±1.12)mV,P<0.05;(4.87±1.37)mV,P<0.01,峰潜时显著减少(49.1±13.4)s,P<0.05;(33.6±12.5)s,P<0.01;与生理盐水组比,1.0,2.0 mg.kg-1的3'-大豆苷元磺酸钠能使眼底荧光血管造影视网膜动脉充盈时间显著减少(48.4±6.7)s,P<0.05;(35.7±3.4)s,P<0.01。结论:3'-大豆苷元磺酸钠对大鼠脑缺血再灌注损伤有明显的保护作用。3'-大豆苷元磺酸钠能扩张视网膜血管,对视网膜缺血再灌注损伤有明显的保护作用。
Objective： To study the effects of 3’-daidzein sulfonate sodium on retina after ischemiareperfusion injury in rats.Method： Rat cerebral ischemia-reperfusion injury modeling was established by blocking arteria cerebri media,the size of cerebral infarction area and the content of brain water were compared before and after sublingual intravenous injection of normal saline,1.0,2.0 mg.kg-1 of 3’-daidzein sulfonate sodium,respectively.Retinal ischemia was induced by clamping the common carotid artery unilaterally.Various doses of 3’daidzein sulfonate sodium（1.0,2.0 mg.kg-1） was injected via the sublingual vein prior to clamping the common carotid artery,and the same volume of normal saline was administered to the model group in rats.The differences of fundus fluorescein angiography and electroretinogram（ERG） at 1 hour were observed before treating and 1 hour after reperfusion.Result： Cerebral infarction area and brain water content of 3’-daidzein sulfonate sodium（1.0,2.0 mg.kg-1） treating groups were significantly lower than it in model group.Compared with normal saline control groups,the amplitude of ERG increased while the peak latencies reduced significantly in 3’-daidzein sulfonate sodium（1.0,2.0 mg.kg-1） treating groups.The 3’-daidzein sulfonate sodium could also significantly shorten the refill time of fundus fluorescein angiography retinal artery.Conclusion： 3’-daidzein sulfonate sodium show significant protect effect on rat cerebral ischemia-reperfusion injury.3’-daidzein sulfonate sodium can greatly dilate retinal vessels and protect it from suffering retinal ischemia-reperfusion injury.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期197-200,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81160399)
