摘要
目的:建立地锦草药材的高效液相指纹图谱,用于评价地锦草药材的质量。方法:采用Promosil C18(4.6 mm×250 mm,5μm)色谱柱,甲醇-0.1%磷酸水为流动相梯度洗脱,流速0.8 mL.min-1,检测波长280 nm,柱温32℃,分析时间85 min。结果:10批地锦草样品得到的高效液相指纹图谱有24个共有峰,各共有峰之间的分离度较好,各批次指纹图谱相似度≥0.90。结论:地锦草药材的指纹图谱特征性及专属性强,可用于地锦草药材的鉴别及质量控制。
Objective： To establish HPLC fingerprint of Herba Euphorbia Humifusae for quality control of the herbal medicine.Method： A Diamonsil C18（4.6 mm × 250 mm,5 μm） analytical column was used.The mobile phase was methanol-0.1% H3PO4 water.The elution was in gradient mode and detection wavelength was set at 280 nm.The column temperature was maintained at 32 ℃ and the flow rate was 0.8 mL.min-1.The analytical time was 60 min.Result： There were 24 common peaks in the fingerprint of 10 batches of Herba Euphorbia Humifusae samples,and each peak in the fingerprint was well separated under the chromatographic condition above.The similarity of the 10 batches of Herba Euphorbia Humifusae was not lower than 0.90.Conclusion： This method is available for the identification and quality control of Herba Euphorbia Humifusae.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期95-98,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
湖南省中医药科技基金重点项目(2009008)
长沙市科技局重点项目(K0802115-31)
