摘要
目的:建立鲜地黄提取物中3种原型入血成分的含量测定方法。方法:以血清药物化学方法追踪确定地黄原型入血成分;采用Inertsil ODS-SP C18色谱柱(4.6 mm×250 mm,5μm),柱温35℃;乙腈-水为流动相,流速1 mL.min-1,检测波长203 nm的HPLC方法,测定鲜地黄提取物中原型入血成分的含量。结果:在大鼠含药血清中发现梓醇、地黄苷D和益母草苷均以原型入血;此3种成分梓醇、地黄苷D、益母草苷线性范围分别为0.32～1.60μg(r=0.999 6),0.406～2.03μg(r=0.999 8),0.8～4.0μg(r=0.999 8);平均回收率分别为99.74%(RSD 2.53%,n=6),96.14%(RSD 1.31%,n=6),100.10%(RSD 2.73%,n=6)。结论:建立的含量测定方法简便可行、重复性良好,测定12批次河南焦作产怀药鲜地黄提取物中此3种成分的含量,总含量最高20.28%,最低11.32%。故建议鲜地黄提取物中3种原型入血成分梓醇、地黄苷D和益母草苷总含量不得少于15%。
Objective： To develop a method for the determination for three prototype constituents into the blood from the extract of Rehmannia glutinosa.Method： The prototype constituents into the blood from R.glutinosa were confirmed by using the method of serum pharmacochemistry,and then the extracts were determined by HPLC.Inertsil ODS-SP C18 column（4.6 mm × 250 mm,5 μm） was used with acetonitrile-water as mobile phase;the column temperature was kept at 35 ℃,and flow rate was 1 mL.min-1.The detection wavelength was set at 203 nm.Result： It was found that in rat serum catalpol,rehmannioside D and leonuride could enter into the blood in prototype forms.The calibration curves of catalpol,rehmannioside D and leonuride were within 0.32-1.60 μg（r = 0.999 6）,0.406-2.03 μg（r = 0.999 8）,0.8-4.0 μg（r = 0.999 8）.The average recoveries were 99.74%（RSD 2.53%）,96.14%（RSD 1.31%） and 100.10%（RSD 2.73%） respectively.Conclusion： The developed methods are simple,feasible and repeatable.They are used to determine three components from 12 batch extracts of R.glutinosa in Jiaozuo district,Henan Province.The maximum total content is 20.28%,and the minimum one is 11.32%.Suggestion that the total content of these three components from the extract of the R.glutinosa must not be less than 15% is provided.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期66-70,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30873442)
国家科技重大专项课题(2011BAI06B02)
关键词
鲜地黄提取物
入血成分
梓醇
地黄苷D
益母草苷
高效液相色谱
extract of fresh Rehmannia glutinosa
content into the blood
catalpol
rehmannioside D
leonuride
HPLC
