摘要
目的:建立高效液相色谱-示差折光检测法(HPLC-RID)测定布洛芬口服液中甘油含量的方法。方法:WatersSpherisorb NH2氨基柱(4.6 mm×250 mm,5.0μm),流动相乙腈-水(85∶15),流速1 mL.min-1,柱温30℃,RID温度35℃。结果:甘油能被有效分离,线性范围0.881 2～88.12μg,检测限和定量限分别为11.05,110.05 ng,加样回收率102.8%(RSD1.4%)。结论:该方法灵敏、简便、重复性好,为布洛芬口服溶液的质量控制提供参考。
Objective： To establish a HPLC-RID method for determination of glycerin in ibuprofen oral solution.Method： Waters Spherisorb NH2 column（4.6 mm × 250 mm,5.0 μm） was adopted with the mobile phase of acetonitrile-water（85 ∶ 15）,column temperature 30 ℃,the flow rate was 1.0 mL.min-1 and RID temperature was 35 ℃.Result： This established method had good linear relationship within the range of 0.881 288.12 μg（r = 0.999 9） for glycerin.The detection and quantitation limits were 11.05,110.05 ng respectively,the average recovery（n = 5） was 102.8%（RSD 1.4%）.Conclusion： This method was convenient,accurate and reliable,which could provide a reference for quality control of ibuprofen oral solution.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期61-63,共3页
Chinese Journal of Experimental Traditional Medical Formulae
