摘要
目的:优选大蒜总皂苷的大孔树脂纯化工艺。方法:以总皂苷转移率为指标,采用单因素试验考察树脂型号、吸附性能(吸附容量、上样液质量浓度、pH、吸附速度)、洗脱性能(洗脱剂浓度、流速、体积),优选大蒜总皂苷纯化工艺。结果:优选的纯化工艺条件为采用AB-8型大孔树脂,树脂-生药1∶1.3,上样液质量浓度0.8 g.mL-1,pH 7,吸附速度2 BV.h-1,加30%乙醇3 BV除杂,95%乙醇5 BV洗脱,洗脱速度3 BV.h-1,大蒜总皂苷纯度>65%。结论:AB-8型大孔树脂可用于纯化大蒜提取物中总皂苷,优选的纯化工艺稳定可行。
Objective： To optimize purification technology of total saponins from Allium sativum with macroporous resin.Method： With transfer rate of total saponins as index,single factor tests were adopted to optimize purification process parameters,such as type of macroporous resin,adsorption capacity of resin（adsorption capacity,the concentration of sample solution,pH and adsorption speed） and elution performance（the concentration,flow rate and volume of eluent）.Result： Optimized purification technology was as following： selected AB-8 macroporous resin,ratio of crude drug-resin 1.3 ∶1,the concentration of sample solution 0.8 g.mL-1,pH 7,adsorption speed 2 BV.h-1,removed impurities with 30% ethanol,eluted with 5 BV of 95% ethanol,elution speed 3 BV.h-1.Under these conditions,purity of total saponins was over 65%.Conclusion： AB-8 macroporous resin could be adopted to purify total saponins from A.sativum,this optimized purification technology was stable and feasible.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期4-7,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重大新药创制科技专项(2009ZX09301-005-11)
关键词
大蒜
总皂苷
大孔树脂
Allium sativum
total saponins
macroporous resin
