摘要
为研究脯氨酸在植物耐盐性和耐热性中的作用,提取菠菜叶片的PSⅡ颗粒,进行高盐(100～800mmol·L-1NaCl)、高温(30和40℃)以及盐和热交叉胁迫处理。PSⅡ最大光化学效率(Fv/Fm)的测定结果表明,0℃下,高盐胁迫处理对PSⅡ颗粒伤害较小;无盐胁迫下,30℃处理对PSⅡ伤害不明显,40℃高温处理则显著降低PSⅡ活性;盐和热交叉胁迫加剧了对PSⅡ的伤害。在PSⅡ颗粒的保存液中加入脯氨酸(100～800mmol·L-1)能显著提高PSⅡ在高盐胁迫以及盐和热交叉胁迫下的活性,但未能提高其在高温胁迫下的活性。这一结果为脯氨酸提高植物耐盐性及对盐和热交叉胁迫抗性方面的功能研究提供了直接证据。
To explore the role of proline in salt tolerance and heat tolerance of plants, photosystem II （PSII） BBY particles prepared from spinach （Spinacia oleracea） leaves were treated with different concentrations of NaC1 （100-800 mmol·L^-1）, different temperatures （30 and 40 ℃ ） and cross treatments of NaC1 and high temper- ature. Then maximal efficiency of PSII photochemistry （Fv/Fm） was determined by plant efficiency analyser （PEA）. The results showed that single salt-treatment caused slight damage to PSII at 0 ℃, and 30 ℃ treatment almost had no damage to PSII under 0 mmol.L1 NaC1 condition, but PSII activity （Fv/Fm） decreased markedly at 40 ℃. While under cross treatments of salt and high temperature, PSII activity decreased more significantly than under the single stress. Addition of 100-800 mmol·L^-1 proline in store buffer could greatly increase the ac- tivity of PSII under salt stress and cross stress of salt and high temperature, but could not increase the activity of PSII under high temperature stress. These results above provide direct evidence that accumulation of proline can enhance salt tolerance and cross-tolerance to salt and high temperature in plants.
出处
《植物生理学报》
CAS
CSCD
北大核心
2013年第6期586-590,共5页
Plant Physiology Journal
基金
作物生物学国家重点实验室开放课题(2011KF07)
江苏省自然科学基金青年基金项目(BK2012073)
关键词
脯氨酸
光系统II
高盐胁迫
高温胁迫
PSII最大光化学效率
proline
photosystem II
high salinity stress
high temperature stress
maximal efficiency of PSIIphotochemistry
