摘要
以海洋鲈鱼为原料,采用胰蛋白酶酶解制备活性肽,以水解度为指标,采用正交试验法对酶解条件进行优化,运用胆酸盐结合试验比较制备的活性肽的体外活性。结果表明,胰蛋白酶酶解鲈鱼蛋白最佳酶解条件为:酶解温度40℃、酶添加量20 000 U/g、pH值8.5、酶解时间5 h,此时水解度可达到12.5%;通过胆酸盐结合试验,发现其体外降血脂活性与酶解时间有关,酶解5 h的酶解液降血脂活性最高(与降血脂药物考来烯胺相当)。
With marine bass as raw material, we used trypsin prepared active peptides. With degree of hydrolysis as the index, orthogonal experimental for enzymatic hydrolysis conditions were optimized, and used cholic acid salt combining experiments to compare the activity of the active peptides in vitro. The results showed that the optimum enzymolysis conditions for marine bass by trypsin were following： the enzymolysis temperature was 40℃, enzyme additives was 20 000 U/g, pH value was 8.5 and the enzymolysis time was 5 h. Under the optimal condition, the degree of hydrolysis reached 12.5%. The result of the cholic acid salt combining experiments showed that the hypolipidemic activity of the active peptides in vitro was related with enzymolysis time. When the enzymolisis time was 5 h, the hypolipidemie activity was the highest, which was closed to eholestyramine.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第9期99-102,共4页
Guangdong Agricultural Sciences
基金
国家科技支撑计划项目(2012BAD33B10
2012BAD37B08)
国家高技术研究发展计划项目(2013AA102201
2013AA102208)
广东省科技计划项目(2011A08043019
2011B010500022
2012A031100013)
关键词
海洋鲈鱼
胰蛋白酶
水解度
降血脂肽
胆酸盐
marine bass
trypsin
degree of hydrolysisi
hypolipidemic peptide
cholic acid salt
