摘要
为筛选胡椒属DNA条形码最佳片段,研究了ITS、rbcL、psbJ-petA和matK基因片段的有效使用性、种内种间变异和barcoding gap,并评估了序列鉴定效率。结果显示:ITS和matK的barcoding gap图相对较好,matK物种水平鉴定成功率高,ITS种间变异较大,而其他2个候选序列不能进行有效鉴定。为此,推荐matK和ITS作为胡椒属植物潜在的DNA条形码序列,并依此探索建立该属的DNA条形码鉴定方法。
In order to screen right DNA regions in Piper, four candidate DNA barcodes with three （rbcL, psbJ-petA, mark） from chloroplast genome and one （ITS） from the nuclear genome, were evaluated in view of 35 self- testing accessions and 282 ones from NCBI. Capability of the four candidate DNA barcodes was evaluated by effectiveness, intra-and inter-specific divergence and barcoding gap analysis, and the identification efficiency was also assessed using Neighbour-Joining method. The results showed that ITS and marK candidate barcodes had clear barcoding gap. At the same time, matK had high species identification reliability, and ITS had high significant divergence at species level. The other two candidate barcodes had no clear barcoding gap. marK and ITS might be the potential DNA barcoding for the identification of Piper plants, thus making the establishment of new identification methods for this genus possible.
出处
《热带作物学报》
CSCD
北大核心
2013年第5期870-874,共5页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31201263)
农业部南亚热作专项(No.12RZZY-13)
