摘要
P6 1蛋白质是从水稻 (OryzasativaL .ssp.japonica)雄性不育突变体农垦 5 8S(NK5 8S)叶绿体中发现的一个蛋白质 ,微序列分析结果表明它的N端氨基酸序列与水稻和大麦叶绿体ATP酶 β亚基的一致。免疫印迹分析证明P6 1与玉米叶绿体ATP酶 β亚基抗体有特异性的交叉反应。P6 1与常规水稻“农垦 5 8”(NK5 8)的 β亚基的分子量基本相同 ,但其等电点偏碱性端 ,与 β相差约 0 .3个pH单位 ,表明P6 1是水稻叶绿体ATP酶 β亚基的变异型 ,是 β亚基的一种同工型 (isoform) ,这里称之为β1。用NK5 8S/NK5 8F2 群体分析β1的遗传分离规律 ,初步证明β1受单隐性核基因调控。
P61 was a protein identified from chloroplasts of Nongken 58S, a male sterile mutant of rice ( Oryza sativa L. ssp. japonica ). Microsequence analysis has revealed that its N_terminal sequence was identical to N_termini of ATPase β subunits of chloroplasts from rice and barley. The antiserum produced using ATPase β subunit from maize specifically recognized P61. P61 had the same molecular weight as the chloroplast ATPase β subunit of wild_type rice “Nongken 58”, but had different isoelectric point (pI) from this β subunit. P61 was more basic than this β subunit. Thus, P61 would be identified as an isoform of the chloroplast ATPase β subunit of rice, named β 1. Genetic analysis with a F 2 population of Nongken 58SדNongken 58” showed that a single recessive genic gene regulated the formation of β 1.
基金
中国科学院重大项目(KY951-A1-302-12-02)
中国科学院生物科学与生物技术研究特别支持费资助项目(STZ-2-09)
国家重点基础研究专项资助项目(G1998010100)
