摘要
以LambdaFIX○RⅡ为载体,构建了盐藻(Dunaliellasalina)的基因组文库。该文库包含了1.1×106个重组子,插入片段平均大小为18kb左右,含插入片段的频率为100%。该文库的容量约为盐藻单倍体基因组的200倍。
A genomic DNA library of Dunaliella salina was constructed using Lambda FIX ○R Ⅱ as vector. The library consisted of 1.1×10 6 clones with an average insert size of about 18 kb. The capacity of this library was about 200 times the equivalent of the haploid genome of D. salina.