摘要
对白沙蒿原生质体培养得到的愈伤组织进行分化诱导 ,得到完整植株 .培养基中 6 BA的浓度是芽分化的关键因素 ,白沙蒿种皮胶质对芽的再生有促进作用 .不同抗菌素对芽分化的作用不尽相同 ,其中头孢霉素可增加芽的分化 ,而卡那霉素则抑制芽的分化 .对白沙蒿种皮胶质进行定性、红外光谱和固体核磁共振分析 ,表明它为纤维素类物质 ,并带有羧基和甲基 .图 4表 4参
Plants were regenerated from protoplast-derived calli in Artemisia sphaerocephala Krasch. 6-BA was indispensable to formation of shoot primordium. Glutinous substance from seed coat of this species had positive effect on shoot regeneration of the calli. Cefotaxime had positive effect on shoot differentiation, while kanamycin exhibited inhibition on it. Analyses of component, IR spectra and 13 C CPMAS NMR spectra revealed that the glutinous substance was an analog of cellulose with methyl and carboxyl group. Fig 4, Tab 4, Ref 15
出处
《应用与环境生物学报》
CAS
CSCD
2000年第1期28-32,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目!(39770 36 5 )
陕西省教委重点科研计划项目!(99JK13)
西北大学科学研究基金资助!(98NW2 5H)
关键词
白沙蒿
种皮胶质
植株再生
抗旱性
愈伤组织分化
antibiotic
Artemisia sphaerocephala Krasch
glutinous substance
plant regeneration
protoplast
structure analysis
