摘要
目的建立测定氟康唑眼用即型凝胶含量的高效液相色谱法,同时测定制剂中氟康唑、氯己定的含量。方法采用ODS-C18色谱柱,以三乙胺缓冲液-甲醇为流动相,流速为1.0 mL/min,检测波长为260 nm。结果氟康唑、氯己定质量浓度线性范围分别为100～600μg/mL(r=0.999 9,n=6)和10～60μg/mL(r=0.999 7,n=6),平均回收率分别为102.78%和101.52%,RSD分别为1.45%和2.70%。结论所用方法操作简便、灵敏度高,重现性好,可用于该制剂的含量测定。
Objective To establish a HPLC method for measuring the content of fluconazole in Situ Forming Eye Gel and simuhaneously determining the content of fluconazole and chlorhexidine in this preparation. Methods The ODS- C18 chromatographic column was used with triethylamine buffer-methanol as the mobile phase,the flow rate was 1.0 mL/min and the detection wavelength was 260 nm. Results The linear ranges of f[uconazole and chlorhexidine were 100- 600 μg/mL（ r = 0.999 9, n = 6） and 10- 60 μg/mL （r =0.999 7, n =6） respectively; the average recovery rates were 102. 78% and 101.52% , RSD 1.45% and 2.70% respective- ly. Conclusion The method is simple to operate and highly sensitive with good reproducibility and can be used for the content deter- ruination of this preparation.
出处
《中国药业》
CAS
2013年第10期74-75,共2页
China Pharmaceuticals
