摘要
目的:探讨白藜芦醇对紫外线诱导人晶状体上皮细胞氧化损伤的保护作用。方法:人晶状体上皮细胞系传代培养,紫外线诱导细胞发生凋亡,20μmol/L白藜芦醇预处理细胞后,观察各项指标改变:流式细胞仪检测细胞凋亡率,比色法检测凋亡相关因子caspase-3及caspase-9的表达,透射电镜观察超微结构变化。结果:流式细胞仪检测发现,白藜芦醇可以抑制紫外线照射诱导的细胞凋亡,阳性对照组中caspase-3及caspase-9含量显著高于同时段阴性对照组,差异有统计学意义(P<0.05);实验组中caspase-3及caspase-9含量均低于同时段阳性对照组,差异有统计学意义(P<0.05)。此外,伴随白藜芦醇作用时间的延长,透射电镜下人晶状体上皮细胞损伤的程度减轻。结论:白藜芦醇可以抑制紫外线诱导的晶状体上皮细胞的凋亡,对辐射性白内障具有预防作用,从而为寻求有效的防治白内障药物提供可靠的实验依据。
AIM： To investigate the protective effect of resveratrol on human lens epithelial cells against ultraviolet-induced apoptosis. METHODS：Subcultured human lens epithelial cell line, ultraviolet induced cell apoptosis, 20μmol/L resveratrol pretreated cell, the indicators change was observed： rate of apoptosis was detected by flow cytometry and apoptosis-related factors of caspses-3 and caspase-9 were detected by colorimetric detection, ultrastructure changes were observed under transmission electron microscope. RESULTS： Flow cytometry instrument testing found that resveratrol can suppress the apoptosis induced by ultraviolet irradiation, caspses-3 and caspase-9 content in positive control group were significantly higher than that of the negative control group at the same time period, the difference was statistically significant （P〈 0.05） ; caspses-3 and caspase-9 content in experimentalgroup were lower than that in the positive control group at the same time, the difference was statistically significant （P〈0.05）. In addition, the damage of human lens epithelial cells was alleviated with the incubation time of resveratrol elongated. CONCLUSION： Resveratrol may inhibit ultraviolet- induced apoptosis of human lens epithelial cells, it has preventive function against radioactive cataract, and it can provide reliable evidence for pursuing effective medicine to prevent and treat cataract.
出处
《国际眼科杂志》
CAS
2013年第6期1073-1076,共4页
International Eye Science
基金
国家自然科学基金(No.30973275)
黑龙江省青年科学基金(No.QC2011C131)~~
关键词
白藜芦醇
紫外线
晶状体上皮细胞
凋亡
resveratrol
ultraviolet light
human lensepithelial cells
apoptosis
