摘要
目的 :建立诃子中 3种可水解丹宁的高效液相色谱测定方法。方法 :色谱柱为NovaPakC18,流动相为甲醇 - 2 %冰醋酸水溶液 (2 7∶73) ,检测波长为 2 80nm ,流速为 1mL·min-1。结果 :3种可水解丹宁诃子酸(chebulinicacid ,Ⅰ ) ,诃黎勒酸 (chebulagicacid ,Ⅱ )和 1,3,6 -三 -O -没食子酰基葡萄糖 (1,3,6 -tri-O -galloyl- β-D - glucose ,Ⅲ )的线性范围分别为 3 32～ 16 6 0 μg ,3 48～ 17 40 μg和 2 74～ 13 70 μg ,回收率分别为 10 0 3% ,94 5 0 %和 85 0 9% ,精密度试验RSD分别为 3 2 % ,3 4%和 3 3%。结论 :该法重现性较好 ,结果可靠 ,可为诃子药材的质量评价提供有效手段。
Objective:A RP-HPLC method was developed for the determination of three hydrolyzable tannins [chebulinic acid(Ⅰ),chebulagic acid(Ⅱ) and 1,3,6-tri- O -galloyl-β- D -glucose(Ⅲ)] in the fruits of Terminalia chebula Retz Methods:Using a NovaPak C 18 column (3 9 mm×150 mm,4 μm) in an oven at 30 ℃,with a solvent system consisting of MeOH-2%HAc(27∶73)and a UV detector at 280 nm, the flow rate was 1 mL·min -1 Results:The linear ranges of Ⅰ～Ⅲ were 3 32～16 60,3 48～17 40 and 2 74～13 70 μg respectively.The average recoveries were 100 3% ( RSD =2 8%, n =5),94 50% ( RSD =2 3%, n =5) and 85 09% ( RSD =4 1%, n =5) respectively The RSD s ( n =5) of measurement precision test were 3 2%,3 4% and 3 3% respectively Conclusion:The method is reproducible, dependable and useful in quality evaluation of the crude drug of Fructus Chebulae
出处
《药物分析杂志》
CAS
CSCD
北大核心
2000年第3期189-191,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家九五攻关项目!"中药材质量标准的规范化研究-诃子"
关键词
诃子
可水解丹宁
反相高效液相色谱
诃子酸
测定
Terminalia chebula Retz , hydrolizable tannins, RP-HPLC, chebulinic acid,chebulagic acid
