摘要
目的:研究脱落酸(Abscisic Acid,ABA)处理对丹参酮类成分积累的影响。方法:用发根农杆菌ATCC15834诱导生产丹参毛状根,经20 d悬浮振荡培养后,分别采用20、90、210、400μmol/L的ABA处理,并用HPLC测定ABA处理7 d后丹参毛状根中丹参酮Ⅰ、隐丹参酮、二氢丹参酮Ⅰ、丹参酮ⅡA的含量。结果:ABA能够抑制丹参毛状根的生长,20μmol/L ABA处理对丹参毛状根生长影响不显著,90、210、400μmol/L的ABA处理条件下,丹参毛状根干重分别减少为对照组的65.2%、71.0%和56.5%;ABA处理能够显著提高丹参毛状根中4种丹参酮的产量,且以210μmol/L ABA处理下达到最高;丹参酮Ⅰ、隐丹参酮、二氢丹参酮Ⅰ和丹参酮ⅡA的含量经ABA处理后分别提高为对照组的5.4、9.5、1.5和1.8倍。结论:ABA能显著促进丹参毛状根中丹参酮类成分的积累。
Objective： To study the effects of Abscisic Acid（ABA） on the accumulation of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots.Methods： After suspension culture of Salvia miltiorrhiza hairy roots induced by agrobacterium rhizogenes ATCC15834 for 20 days,the ABA was added into suspension cultures,the contents of tanshinones（including tanshinoneⅠ,cryptotanshinone,dihydrotanshinonⅠand tanshinone ⅡA）were detected by HPLC after 7 days.Results： The growth of Salvia miltiorrhiza hairy roots was inhibited by ABA.20 μmol/L ABA treatment had no significant effect.The dry weight of Salvia miltiorrhiza hairy roots of other ABA treatments reduced to 65.2%,71% and 56.5% of the control,respectively.Different levels of ABA could significantly increased the yields of four tanshinones in Salvia miltiorrhiza hairy roots.The yields of tanshinoneⅠ,cryptotanshinone,dihydrotanshinonⅠand tanshinoneⅡA were significantly increased to 5.4,9.5,1.5 and 1.8 times that of the control group after dealing with 210 μmol/L ABA.Conclusion： ABA can stimulate the accumulation of tanshinones in Salvia miltiorrhiza hairy roots.
出处
《中药材》
CAS
CSCD
北大核心
2013年第3期354-358,共5页
Journal of Chinese Medicinal Materials
关键词
脱落酸
丹参毛状根
丹参酮积累
ABA
Salvia miltiorrhiza hairy roots
Tanshinones accumulation
